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Cell removal from fermentation broth by flocculation + sedimentation

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Summary

Cell separation by flocculation+sedimentation ofStreptoccocus equisimilis cultivation for hyaluronate lyase recovery, was investigated as a function of the pH of the fermentation broth, using three different cationic flocculants. The polyelectrolyte Superfloc N-100 appears to be the best of the three flocculants tested; after treatmen of pH 6.0 and 120 min free sedimentation, the cells are sedimented at 20% of the initial volume and 80% of the volume remained as a clear supernatant without loss of enzyme activity.

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Author notes

  1. Chico E.

    Present address: Cyanamid Iberica S. A., Madrid, Spain

Authors and Affiliations

  1. Departamento de Bioquímica, Facultad de Farmacia, Universidad de Sevilla, Spain

    Bautista J., Chico E. & Machado A.

Authors
  1. Bautista J.
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  2. Chico E.
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  3. Machado A.
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Bautista, J., Chico, E. & Machado, A. Cell removal from fermentation broth by flocculation + sedimentation. Biotechnol Lett 8, 315–318 (1986). https://doi.org/10.1007/BF01040856

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  • DOI: https://doi.org/10.1007/BF01040856

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