Summary
Germination of condiospores fromA.niger in very concentrated suspension was required to inoculate solid fermentation media, but a germination self-inhibitor was detected in spores. It was found that the inhibitory effect depended on the composition of the medium and was reduced when glucose was used as a carbon source. The effect of the self-inhibitor was eliminated by washing the spores and using glucose and a protein nitrogen source in the germination medium. By this method it was possible to increase about 100 times (106 to 108) spore concentration, keeping more than 90% germination.
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Barrios-González, J., Martínez, C., Aguilera, A. et al. Germination of concentrated suspensions of spores from Aspergillus niger. Biotechnol Lett 11, 551–554 (1989). https://doi.org/10.1007/BF01040034
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DOI: https://doi.org/10.1007/BF01040034