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Effect of water activity on enzymatic synthesis of cephalexin

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Summary

In enzymatic synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and D-α-phenylglycine methyl ester (PGM) using an acylase fromXanthomonas citri, it was found that the synthetic activity and conversion yield were enhanced markedly by depressing the water activity (a w ) of reaction system. This enhancement was probably resulted from the change of thermodynamic equilibrium and maximized at a range ofa w from 0.96 to 0.97.

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Authors and Affiliations

  1. Department of Biological Science and Engineering, Korea. Advanced Institute of Science and Technology, Cheongyang, P.O.Box 150, 130-650, Seoul, Korea

    Chang K. Hyun, Jung H. Kim & Young J. Kim

  2. Central Research Institute, Korea Steel Chemical Co., Ltd., 461-120, Sungnam, Korea

    Young J. Kim

Authors
  1. Chang K. Hyun
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  2. Jung H. Kim
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  3. Young J. Kim
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Hyun, C.K., Kim, J.H. & Kim, Y.J. Effect of water activity on enzymatic synthesis of cephalexin. Biotechnol Lett 11, 537–540 (1989). https://doi.org/10.1007/BF01040031

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