Summary
A pleiotropic mutation in thepurB gene ofE. coli is described which lowers the spontaneous mutation frequency of other genes. The antimutator effect is very large for some genetic loci, but is absent at other sites. Both forward and reverse mutations are affected. This mutation inpurB is temperature sensitive for both adenine auxotrophy and the antimutator action. Adenine, or adenosine, or low temperature growth abolish the antimutator effect. The mutagenicity of base analogs and nitrosoguanidine at several loci was found to be reduced by thispurB mutation. The antimutator effect is recessive in strains merodiploid for thepurB region. The frequency of reversion of mutation on F′ episomes is affected by the chromosomal antimutator, which therefore acts in trans. Xray and UV sensitivity are normal in this mutant, which is the first antimutator characterized inE. coli.
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Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map ofEscherichia coli K-12. Bact. Rev.40, 116–167 (1976)
Bazzicalupo, P., Tocchini-Valentini, G.P.: Curing of anEscherichia coli episome by rifampicin. Proc. nat. Acad. Sci. (Wash.)69, 298–300 (1972)
Bonhoeffer, F., Schaller, H.: A method for selective enrichment of mutants based on the high UV sensitivity of DNA containing 5-bromouracil. Biochem. biophys. Res. Commun.20, 93–97 (1965)
Chopra, V.L.: Gene controlled change in mutational stability of a tryptophanless mutant ofE. coli WP2., Mutation Res.4, 382–384 (1967)
Chu, E.H.Y., Sun, N.C., Chang, C.C.: Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light. Proc. nat. Acad. Sci. (Wash.)69, 3459–3463 (1972)
Clarke C.H., Shankel, D.M.: Antimutagenesis in microbial systems. Bact. Rev.39, 33–53 (1975)
Coukell, M.B., Yanofsky, C.: Increased frequency of deletions in DNA polymerase mutants ofEscherichia coli. Nature (Lond.)228, 633–635 (1970)
Demerec, M.: Frequency of spontaneous mutations in certain stocks of Drosophila melanogaster. Genetics22, 469–478 (1937)
DeSerres, F.J.: Mutability of sensitive strains ofNeurospora crassa. Genetics68, S14 (1971)
Drake, J.W., Allen, E.F.: Antimutagenic DNA polymerase of bacteriophage T4. Cold Spr. Harb. quant. Biol.33, 339–344 (1968)
Drake, J.W., Greening, E.O.: Suppression of chemical mutagenesis in bacteriophage T4 by genetically modified DNA polymerase. Proc. nat. Acad. Sci. (Wash.)66, 823–829 (1970)
Esposito, M.S., Bolotin-Fukuhara, M., Esposito, R.E.: Antimutator activity during mitosis by a meiotic mutant of yeast. Molec. gen. Genet.139, 9–18 (1975)
Gross, J.D., Karamata, D., Hempstead, P.G.: Temperature-sensitive mutants ofB. subtilis defective in DNA synthesis. Cold Spr. Harb. Symp. iquant. Biol.33, 307–312 (1968)
Hall, R.M., Brammar, W.J.: Increased spontaneous mutation rates in mutants ofE. coli with altered DNA polymerase III. Molec. gen. Genet.121, 271–276 (1973)
Hershfield, M.S.: On the, role of deoxyribonucleic acid polymerase in determining mutation rates. J. biol. Chem.248, 1417–1423 (1973)
Low, K.B.:Escherichia coli K-12 F-prime factors, old and new. Bact. Rev.36, 587–607 (1972)
Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory, 1972
Mohn, G., Wurgler, F.E.: Mutator genes in different species. Humangenetik16, 49–58 (1972)
Mount, D.W., Low, K.B., Edmiston, S.J.: Dominant mutations (lex) inEscherichia coli (K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J. Bact.112, 886–893 (1972)
Pledger, W.J., Umbarger, H.E.: Isoleucine and valine metabolism inEscherichia coli: XXI. Mutations affecting derepression and aline resistance. J. Bact.114, 183–194 (1973)
Siegel, E.C.: Ultraviolet-sensitive mutator strain ofEscherichia coli K-12. J. Bact.113, 145–160 (1973a)
Siegel, E.C., Ivers, J.J.:mut-25, a mutation, to mutator linked topurA inEscherichia coli. J. Bact.121, 523–530 (1975)
Speyer, J.F.: Mutagenic DNA polymerase. Biochem. biophys. Res. Commun.21, 6–8 (1965)
Speyer, J.F.: Base analogues and DNA polymerase mutagenesis. Fed. Proc.28, 348 (1969)
Vaccaro, K.K., Siegel, E.C.: Increased spontaneous reversion of certain frameshift mutations in DNA polymerase deficient strains of Escherichia coli. Molec. gen. Genet.141, 251–262 (1975)
Zamenhof, S., Heldenmuth, L.H., Zamenhof, P.J.: Studies on mechanisms for the maintenance of constant mutability: Mutability and the resistance to mutagens. Proc. nat. Acad. Sci. (Wash.)55, 50–58 (1966)
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Communicated by Ch. Auerbach
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Geiger, J.R., Speyer, J.F. A conditional antimutator inE. coli . Molec. Gen. Genet. 153, 87–97 (1977). https://doi.org/10.1007/BF01036000
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DOI: https://doi.org/10.1007/BF01036000