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Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques

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Summary

The fixation and drying regimes for frozen sections and cytocentrifuge preparations for the demonstration of surface antigens, such as immunoglobulins and iron binding proteins, vary enormously between different groups of workers. A method using freeze-dried sections and acetone fixation was compared with 16 other methods of fixation and found to be the best for tissue preservation and antigen demonstration. Freeze drying was found to improve the cytological preservation of air-dried sections considerably.

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Authors and Affiliations

  1. Department of Experimental Pathology, Southampton General Hospital, Southampton University, Tremona Road, SO9 4XY, Southampton, UK

    Mary A. Judd & Karen J. M. Britten

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  1. Mary A. Judd
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  2. Karen J. M. Britten
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Judd, M.A., Britten, K.J.M. Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques. Histochem J 14, 747–753 (1982). https://doi.org/10.1007/BF01033624

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  • DOI: https://doi.org/10.1007/BF01033624

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