Abstract
Trimethyl and triethyl esters of tricarballylic acid and citric acid were hydrolysed with porcine liver esterase(PLE) to the isomeric diesters. In all cases the hydrolysis took place with poor regioselectivity (maximum 50% excess). However, the hydrolysis of trimethyl and triethyl esters of tricarballylic acid and of the triethyl ester of citric acid with subtilisin was absolutely regioselective and the symmetric 1,5-diester was obtained.
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Kvittingen, L., Partali, V., Brænden, J.U. et al. Highly regioselective hydrolysis of tricarballylates (“retro-fats”) and citrates by subtilisin. Biotechnol Lett 13, 13–18 (1991). https://doi.org/10.1007/BF01033509
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DOI: https://doi.org/10.1007/BF01033509