Skip to main content
SpringerLink
Log in

Resolution of the extracellular α-glucosidase system ofBacillus sp. NCIB 11203

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Two extracellular α-glucosidases (EC 3.2.1.20, α-D-glucoside glucohydrolase) of the alkalophilic bacterium,Bacillus sp. NCIB 11203, were separated, purified and partially characterised. Resolution of the system into two separate enzymes was achieved by fractionation with (NH4)2SO4 and chromatography on DEAE-Biogel A. The first of these activities, an α-glucosidase, hydrolysed p-nitrophenyl-α-D-glucopyranoside preferentially and had minor activity on isomaltose and isomaltotriose. The second enzyme was a maltase and displayed highest activity on maltose and maltotriose and some activity on p-nitrophenyl-α-D-glucopyranoside.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boyer, E.W. and Ingle, M.B. (1972). J. Bacteriol.110:992–1000.

    Google Scholar 

  • Fogarty, W.M. (1983). Microbial amylases.In Microbial enzymes and biotechnology. Chap. 1.Edited by W. M. Fogarty. Applied Science Publishers, London. pp. 1–92.

    Google Scholar 

  • Fogarty, W.M., Kelly, C.T. and Kadam, S.K. (1985). Can. J. Microbiol.31: 670–674.

    Google Scholar 

  • Huggett, A.S.C. and Nixon, D.A. (1955). Biochem. J.66: 12–19.

    Google Scholar 

  • Kelly, C.T. and Fogarty, W.M. (1983). Process Biochem.18: 6–12.

    Google Scholar 

  • Kelly, C.T., Giblin, M. and Fogarty, W.M. (1986). Can. J. Microbiol.32: 342–347.

    Google Scholar 

  • Kelly, C.T., Moriarty, M. and Fogarty, W.M. (1983a). Biotechnol. Lett.5: 289–294.

    Google Scholar 

  • Kelly, C.T., O'Reilly, F. and Fogarty, W.M. (1983b). FEMS Microbiol. Lett.20: 55–59.

    Google Scholar 

  • Lowry, D.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). J. Biol. Chem.193: 265–275.

    Google Scholar 

  • Thirunavukkarasu, M. and Priest, F.G. (1984). J. Gen. Microbiol.130: 3135–3141.

    Google Scholar 

  • Urlaub, H. and Wöber, G. (1978). Biochim. Biophys. Acta,522: 161–173.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Industrial Microbiology, University College, Dublin 4, Ireland

    C. T. Kelly, P. A. Brennan & W. M. Fogarty

Authors
  1. C. T. Kelly
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. A. Brennan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. M. Fogarty
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kelly, C.T., Brennan, P.A. & Fogarty, W.M. Resolution of the extracellular α-glucosidase system ofBacillus sp. NCIB 11203. Biotechnol Lett 9, 125–130 (1987). https://doi.org/10.1007/BF01032751

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01032751

Keywords

Search

Navigation