Summary
Aspergillus niger glucoamylase was adsorbed to β-naphthyl cotton cloth by hydrophobic interaction. The adsorbed enzyme was cross-linked with glutaraldehyde. The immobilized glucoamylase exhibited greater pH dependence though the optimal pH did not change. The immobilized glucoamylase in a packed bed column completely hydrolysed 5% soluble starch at a specific velocity of about 4. Used naphthyl cloth could be regenerated by heating in 2 N NaOH at 100°C for 1 hour.
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References
Miller, G.L. (1959). Anal. Chem. 31, 426–428.
Sharma, S., and Yamazaki, H. (1984). Biotechnology Letters 6, 301–306.
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Sharma, S., Yamazaki, H. Immobilization of glucoamylase on naphthyl cotton cloth. Biotechnol Lett 7, 769–772 (1985). https://doi.org/10.1007/BF01032294
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DOI: https://doi.org/10.1007/BF01032294