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Continuous cultivation of hybridoma cells in cell reactor

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Summary

Mouse hybridoma cells were grown on surface of glass disks in a cell reactor. It is shown that during continuous cultivation the cells retain their antibody-producing ability. The yield of the culture supernatant obtained in the reactor exceeds about 5 fold those obtained in flasks with equivalent area.

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Authors and Affiliations

  1. Department of Cell Biophysics, Institute of Biological Physics of the USSR Academy of Sciences, Pushchino, Moscow Region, USSR

    Vyacheslav P. Pankratov, Yuri A. Mantzygin, Oleg S. Morenkov & Natal'ya V. Swyatukhina

Authors
  1. Vyacheslav P. Pankratov
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  2. Yuri A. Mantzygin
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  3. Oleg S. Morenkov
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  4. Natal'ya V. Swyatukhina
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Pankratov, V.P., Mantzygin, Y.A., Morenkov, O.S. et al. Continuous cultivation of hybridoma cells in cell reactor. Biotechnol Lett 7, 141–146 (1985). https://doi.org/10.1007/BF01027807

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  • DOI: https://doi.org/10.1007/BF01027807

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