Summary
Myrosinase, purified from white mustard seeds by affinity chromatography and homogeneous in SDS-PAGE, was used in immobilization experiments on common inexpensive solid matrices viz. trimaleylchitosan, gamma-alumina, gamma-alumina activated by dodecadiamin, silanized sand, silanized silica and cellulose triacetate. For each matrix the absorption and immobilization yields at pHs 5.5 and 7.2 were determined. For these parameters, gamma-alumina showed the most interesting results. The maximum amount of enzyme immobilized was ca. 30 mg/g of gamma-alumina. The enzyme also showed good static and operational stability.
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Iori, R., Leoni, O. & Palmieri, S. Immobilization of myrosinase (Thioglucoside glucohydrolase EC 3.2.3.1). Biotechnol Lett 10, 575–578 (1988). https://doi.org/10.1007/BF01027132
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DOI: https://doi.org/10.1007/BF01027132