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Production of the recombinant p36 protein from HIV-2 inEscherichia coli using the trp promoter

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Summary

The effect of several parameters on the production of a recombinant protein (p36 of HIV-2) was studied. The expression level achieved depended on the host strain and the induction conditions. The highest level of expression of p36 (20%) was obtained when the trytophan promoter was inducer by derrepression, using 5 ug/ml of tryptophan, without 3-β Indoleacrylic Acid.

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Authors and Affiliations

  1. Division of Immunotechnology and Diagnostics, Center for Genetic Engineering and Biotechnology, Cubanacan, P.O.Box 6162, Havana, Cuba

    R. E. Narciandi & A. Delgado

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  1. R. E. Narciandi
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  2. A. Delgado
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Narciandi, R.E., Delgado, A. Production of the recombinant p36 protein from HIV-2 inEscherichia coli using the trp promoter. Biotechnol Lett 14, 1103–1108 (1992). https://doi.org/10.1007/BF01027010

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