Summary
Anaerobic growth ofCellulomonas uda on cellobiose was investigated. With uncontrolled pH, a total metabolic inhibition due to the combined effect of low pH and acid accumulation occurred after 2 g/l cellobiose had been used. This inhibition was partly reduced when the culture pH was maintained at 7.0, but then the growth rate and growth yield were found to be both reduced. Endoglucanase synthesis was related to the cellobiose concentration under these batch growth conditions and markedly influenced by the dilution rate in chemostat.
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References
Bagnara,C., Gaudin,C., Belaich,JP., (1987) Appl.Microbiol.Biotechnol.26:170–176.
Bensadoun, A., Weinstein, D., (1976) Anal. Biochem. 70, 241–250.
Clemmer,J.E., Tseng,C.H., (1986) Biotechnol. Letters.8,11.,823–826
Dermoun,Z., Belaich,J.P., (1988) Appl.Microbiol.Biotechnol. 27,399–404.
Giallo, J., Gaudin, C., Belaich, J.P., Petitdemange, E., Caillet-Mangin,F. (1988) Appl. Environ. Microbiol., 45:843–849.
Holdeman,L.V., Cato,E.P., Moore,W.E.C.,(1977) Anaerobic Laboratory Manual 4°ed., Virginia Polytechnic Institute.ed:Moore, W.E.C.
Lang, E., Lang, H., (1972) Anal. Chem., 260, 8–10.
Park, J.T., Johnson, M.J., (1949) J. Biol. Chem., 181, 149–151.
Stackebrandt,E., Haringer,M., Schleifer,K.H., (1980). Arch.Microbiol.,127,179–185
Stoppok,W., Rapp,P., Wagner,F., (1982) Appl. Environ. Microbiol., 44, 44–53
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Dermoun, Z., Belaich, J.P. Effects of cellobiose concentration on the anaerobic growth ofCellulomonas uda . Biotechnol Lett 10, 365–368 (1988). https://doi.org/10.1007/BF01026167
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DOI: https://doi.org/10.1007/BF01026167