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Effect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cells

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Abstract

Hybridoma cells (S3H5/γ2bA2) are found to grow either in suspension or as attached to the surface of cell culture T flask. Cell growth rates and monoclonal antibody (MAB) production rates of both suspended and attached cells were examined. Although the percentage of viable cells was higher for the attached cells, cells growing in suspension showed almost the same charateristics as cells attached to the flasks with respect to cell growth and MAB production rate. Cell attachment increased with increasing serum concentrations up to 5% and remained essentially constant at cell densities of about 2·105/cm2.

No differences in cell growth rate and MAB production could be attributed to anchorage dependent growth.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Michigan, 48109, Ann Arbor, MI

    Gyun Min Lee & Bernhard O. Palsson

  2. Department of Internal Medicine, University of Michigan, 48109, Ann Arbor, MI

    Thomas K. Huard

Authors
  1. Gyun Min Lee
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  2. Thomas K. Huard
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  3. Bernhard O. Palsson
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Lee, G.M., Huard, T.K. & Palsson, B.O. Effect of anchorage dependency on growth rate and monoclonal antibody production of hybridoma cells. Biotechnol Lett 10, 307–312 (1988). https://doi.org/10.1007/BF01026156

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  • DOI: https://doi.org/10.1007/BF01026156

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