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Ribitol dehydrogenase overproduction and maintenance of plasmids pBR322 and pACYC184 in chemostat cultures of Escherichia coli

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The maintenance of multicopy plasmids pBR322 and pACYC184 was studied in chemostat cultures subjected to limitation by glucose, ribitol or xylitol at D of 0.1 per h. While carbon source-dependent segregational stability of pBR322 was observed, no dependence and greater instability of pACYC184 was found. Plasmid-free and plasmid-bearing strains overproducing chromosomally coded ribitol dehydrogenase were found in pentitol-limited chemostat cultures.

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Authors and Affiliations

  1. Department of Enzyme Engineering, Institute of Microbiology CAS, Vídeňská 1083, 142 20, Praha 4

    P. Kyslík, L. Sobotková, B. Večerek, M. Dobišová & T. Chrudimský

Authors
  1. P. Kyslík
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  2. L. Sobotková
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  3. B. Večerek
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  4. M. Dobišová
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  5. T. Chrudimský
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Kyslík, P., Sobotková, L., Večerek, B. et al. Ribitol dehydrogenase overproduction and maintenance of plasmids pBR322 and pACYC184 in chemostat cultures of Escherichia coli. Biotechnol Lett 11, 149–154 (1989). https://doi.org/10.1007/BF01026047

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  • DOI: https://doi.org/10.1007/BF01026047

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