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Enzyme sensor for pullulan and pullulanase activity

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Summary

An bienzyme sensor consisting of coimmobilized glucoamylase and glucose oxidase attached to the tip of a modified oxygen electrode was applied to determine pullulan (linear range: 0.001 to 0.01 %) within 90 sec or soluble pullulanase (linear range: 0.05 to 0.7 U/ml).

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References

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Authors and Affiliations

  1. Central Institute of Molecular Biology, Dept. Applied Enzymology, Academy of Sciences of the GDR, Robert-Rössle-Str. 10, 1115, Berlin, GDR

    R. Renneberg, G. Kaiser & F. Scheller

  2. Hayashibara Biochemical Laboratories, Inc., 2-3 Shimoishii 1-chome, 700, Okayama, Japan

    Y. Tsujiseka

Authors
  1. R. Renneberg
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  2. G. Kaiser
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  3. F. Scheller
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  4. Y. Tsujiseka
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Renneberg, R., Kaiser, G., Scheller, F. et al. Enzyme sensor for pullulan and pullulanase activity. Biotechnol Lett 7, 809–812 (1985). https://doi.org/10.1007/BF01025559

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  • DOI: https://doi.org/10.1007/BF01025559

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