Abstract
A chemical modification of the gene 5 DNA binding protein (G5BP) from bacteriophage fd was investigated using X-ray diffraction and difference Fourier analysis. The crystalline protein was reacted with pentaammineruthenium (III) trichloride, Ru(NH3)5Cl3, a reagent believed specific for histidine residues and useful in NMR and chemical modification studies of proteins. The major ruthenium site was found by difference Fourier analysis to be 4 Å from histidine 64, the only histidine residue in the molecule. A second bipartite site, believed to be a ruthenium-anion pair, appeared to be salt-bridged to glutamic acid 40 and arginine 16. Indications were present in the difference Fourier results to suggest that the β loop containing tyrosine 41 had undergone a slight conformational rearrangement to accommodate this interaction.
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References
Brayer, G. D., and McPherson, A. (1983).J. Mol. Biol. 169, 565–596.
Brayer, G. D., and McPherson, A. (1984a).Biochemistry 23, 340–349.
Brayer, G. D., and McPherson, A. (1984b).J. Biomol. Struct. Dynam. 2, 495–510.
Clarke, M. J. (1980a). InMetal Ions In Biological Systems (Siegel, H., ed.), Vol. II, Marcel Dekker, New York.
Clarke, M. J. (1980b). InInorganic Chemistry in Biology and Medicine, ACS Symposium Series 140, pp. 157–180.
Diamond, S. E., and Taube, H. (1975).J. Am. Chem. Soc. 97, 5921.
Matthews, C. R., Recchia, J., Froebe, C. L. (1981).Analytical Biochemistry 112, 329–337.
Matthews, C. R., Erickson, P. M., and Froebe, C. L. (1980).Biochim. Biophys. Acta 624, 499–510.
McPherson, A., Molineux, I., and Rich, A. (1976).J. Mol. Biol. 106, 1077–1081.
Recchia, J., Matthews, C. R., Rhee, M.-J., and Horrucks, W. D., Jr. (1982).Biochim. Biophys. Acta 702, 105–111.
Rosenberg, B., Rensrow, L., van Camp, J., Hartwick, J., and Drobnik, J. (1967).J. Bacteriol. 93, 716.
Rubin, J. F., Sabat, M., and Sundaralingham, M. (1983).Nucleic Acids Res. 11, 6571–6586.
Vogt, L. H., Katz, J. L., and Wimberley, S. E. (1965).Inorg. Chem. 1, 1157.
Waysbort, D., Evenor, M., Navon, G. (1975).Inorg. Chem. 14, 514–519.
Winkler, J. R., Nocera, D. G., Yocom, K. M., Brodignon, E., and Gray, H. B. (1982).J. Am. Chem. Soc. 104, 5800–5801.
Yocom, K., Shelton, J. B., Shelton, J. R., Schroeder, W. A., Worosila, G., Isled, S. S., Brodignon, E., and Gray, H. B. (1982).Proc. Natl. Acad. Sci. USA 79, 7052–7055.
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Axelrod, H., DeLozier, G., Greene, S. et al. Modification of the gene 5 DNA unwinding protein with ruthenium. J Protein Chem 4, 235–243 (1985). https://doi.org/10.1007/BF01025301
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DOI: https://doi.org/10.1007/BF01025301