Summary
Two β-glucosidase genes, designatedbglA andbglB, were isolated from a gene bank ofClostridium thermocellum DSM 1237. The coding sequences forbglA andbglB were located on non-homologous DNA fragments of 3.2− and 3.4-kb, respectively. Both genes direct inEscherichia coli the synthesis of cytoplasmic β-glucosidases, which differ with respect to substrate specificity and temperature profile. The properties of thebglA-encoded β-glucosidase A closely resemble that of a β-glucosidase previously isolated fromC. thermocellum cultures.
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Gräbnitz, F., Staudenbauer, W.L. Characterization of two β-glucosidase genes fromClostridium thermocellum . Biotechnol Lett 10, 73–78 (1988). https://doi.org/10.1007/BF01024629
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DOI: https://doi.org/10.1007/BF01024629