Summary
Accomparative study of six hydrolases, acid and alkaline phosphatases, aryl sulphatase, β-gluchronidase cholinesterase, and non-specific esterase, was carried out on the tissues of normal healthy and Frescon-treatedBulinus. The presence and activity of these enzymes in the tissues of normal animals were taken to indicate the probale functions of the tissues concerned. Frescon administration caused inhibition of acid phosphatase and also induced the release of cholinesterase and non-specific esterase in some tissue. It is concluded that the most important effects of Frescon on snail physiology are the disorganization of neuronal function and disturbance of olfactory activity.
Similar content being viewed by others
References
Banna, H. B. (1977)The general histology and histopathology of Bulinus truncatus (Audouin), with special reference to the action of a molluscicide. Ph.D. Thesis, London University.
Barka, T. &Anderson, P. J. (1963).Histochemistry. New York: Harper and Row.
Bauffard, T. G. (1970) Functional histology and histochemistry of the gill and the nervous system of the mussel,Mutilus edulis. Biol. 2477B–2478B.
Dabq, M. (1962) Localizations et evolution des phosphatases aux premier staesidu developement.Bull. Acad. r. Med. Belg. 7, 573–610.
Dalq, A. M. (1963) The relation to lysosomes of thein vivo metachromatic granules. InLysosomes (edited byDe Reuck, A. V. andCameron, M. P.), Ciba Symposium. London: Churchill.
Demal, J. (1955) Essai d'histologie comparee des organs chemorecepteur des gastropodes.Acad. r. Belg. 29, 1–83.
Giller, E. &Schwartz, J. (1970). Acetylcholinesterase in identified neurons of abdominal ganglion inAplysia californica.J. Neurophysiol 34, 108–15.
Gomori, G. (1950)Microscopic Histochemistry. Chicago University Press.
Holt, S. J. (1958) Indigogenic staining methods for esterases. InGeneral Cytochemical Methods (edited byDanielli, J. F.). Academic Press: New York.
Jurchenko, O. P., Kultas, K. N. &Vulfius, C. A. (1973) Cholinesterase activity in ganglia of Gastropoda,Lymmeaes stagnalis andPlanorbarius corneus. II. Histochemical investigation.Comp. Biochem. Physiol. 45A, 61–8.
Korn, E. (1969) Cholinesterase activity in the tissues of the snailHelix aspersa.Comp. Biochem. Physiol. 28, 923–9.
Lake, E. D. (1966) The histochemistry of phosphatases. The use of lead acetate instead of lead nitrate.Jl R. microsc. Soc. 83, 73–4.
Moore, M. N. &Halton, D. W. (1973) Histochemical changes in the digestive gland ofLymnaea trunculata, infected withFasciola hepatica.Z. Parasitenk. 43, 1–16.
Moore, M. N. &Stebbing, A. R. D. (1976) The quantitative cytochemical effects of three metal ions on a lysosomal hydrolase of a hydroid.J. mar. biol. Ass. U.K. 56, 995–1005.
Pearse, A. G. E. (1968)Histochemistry, Theoretical and Applied Vol. I. London: Churchill Lyvingstone.
Pearse, A. G. E. (1972).Histochemistry, Theoretical and Applied. Vol. II. London: Churchill Livingstone.
Pearson, C. K. (1963) A formalin-sucrose ammonia fixation for cholinesterases.J. Histochem. Cytochem. 11, 665–6.
Plummer, J. M. &Banna, H. B. (1979) Activity changes in some large neurons ofArchachatina caused by the molluscicide Frescon.Comp. Biochem.Physiol. 62C, 9–18.
Rosca, D. I. &Dordea, M. (1971) Studies on the functional role of acetylcholinesterase in osmoregulation inHirudo medicinalis.Studia. Univ. Babes-Bolyai. 16, 119–22.
Rutenberg, A. M., Cohen, R. B. &Seligman, A. M. (1952) Histochemical demonstration of aryl sulphatases.Science, N.Y. 116, 539–43.
Shell Chemicals Handbook (1974) Frescon: A molluscicide for the better control of Schistosomiasis. Shell Chemicals Ltd.
Silver, A. (1974) The biology of cholinesterases. InFrontiers of Biology (edited byNeuroberger, A. andTatum, E.), 36, Amsterdam: North Holland.
Suzuki, S., Takahashi, N. &Egam, F. (1957) Enzymatic transsulfation from a phenol to carbohydrates.Biochem. Biophys. Acta 24, 444–5.
Wagge, L. E. (1951) The activity of amoebocytes and of alkaline phosphatases during the regeneration of the shell in the snail,Helix aspersa.Q. Jl microsc. Sci. 92, 307–21.
W. H. O. (1965) Snail control in the prevention of Bilharziasis. Monograph series no. 50, Geneva.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Banna, H.B.M. Histochemical studies of some enzymes in the tissues of the schistosome vector snailBulinus truncatus (Audouin) with special reference to the effects of a molluscicide. II. Hydrolases. Histochem J 12, 145–152 (1980). https://doi.org/10.1007/BF01024545
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01024545