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Ultrastructural, histochemical, and biochemical damage induced by chlorpyrifos in the midgut of Chrysomya megacephala larvae after acute exposure

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Abstract

The concentration–mortality response of third instar larvae of Chrysomya megacephala (Diptera: Calliphoridae) to a synthetic insecticide, chlorpyrifos, and its impact on ultrastructure and carbohydrate and protein histochemistry were determined in laboratory assays. Toxic effects of chlorpyrifos on the nutritional parameters (carbohydrates, proteins, lipids, and cholesterol) and the activities of acid phosphatase and alkaline phosphatase were also studied through biochemical assays. Larvae displayed a concentration and time-dependent mortality response for the insecticide. The midgut was analyzed by transmission electron microscopy, which demonstrated ultrastructural alterations in nuclei, lipid spheres, microvilli, mitochondria, rough endoplasmic reticulum lysosomes. Histochemical tests revealed a strong reaction for proteins and carbohydrates in the control group and a weak reaction in the group exposed to chlorpyrifos. Chlorpyrifos also caused a significant reduction in the total midgut content of carbohydrates, proteins, lipids, and cholesterol. Tested insecticide also reduced the activities of acid and alkaline phosphatases at all concentrations compared to untreated larvae. The effects were concentration and time-dependent.

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  1. Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Shagufta Yasmeen & Mohammad Amir

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Yasmeen, S., Amir, M. Ultrastructural, histochemical, and biochemical damage induced by chlorpyrifos in the midgut of Chrysomya megacephala larvae after acute exposure. Int J Trop Insect Sci 42, 3793–3806 (2022). https://doi.org/10.1007/s42690-022-00904-5

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