Summary
Pertussis toxin subunits (S1–S5) were expressed inBacillus subtilis using a vector with the promoter, Shine-Dalgarno sequence and the sequence coding for the first 7 amino acids of the signal sequence of the α-amylase gene fromB. amyloliquefaciens. The use of this vector resulted in a high level of intracellular expression of each pertussis toxin subunit as aggregates in the cytoplasm ofB. subtilis.
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Saris, P.E.J., Airaksinen, U., Nurmiharju, S. et al. Expression ofBordetella pertussis toxin subunits inBacillus subtilis . Biotechnol Lett 12, 873–878 (1990). https://doi.org/10.1007/BF01022582
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DOI: https://doi.org/10.1007/BF01022582