Synopsis
Cytochemical studies on the localization and substrate specificities of acid phosphatase activities in the epithelial cells of the midgut ofCarausius morosus have revealed the presence of two distinct types of phosphatases. Acid naphthol AS-BI phosphatase activity was present at particulate (lysosomal) sites in all regions of the midgut and its activity was particularly high in the pear-shaped organs. Acid β-glycerophosphatase of low activity was present in the mid and posterior midgut regions, but was absent from the pearshaped organs. In the anterior region of the midgut, acid β-glycerophosphatase activity could only be found associated with the concentrically laminated vesicles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berkaloff, M. a. (1959). Les grains de secretion des tubes de Malpighi deGryllus domesticus.C. R. Acad. Sci. 246, 2807–9.
Bitensky, L. (1962). The demonstration of lysosomes by controlled-freeze-sectioning method.Q. Jl microsc. Sci. 103, 205–9.
Bowen, I. d. (1968). Electron cytochemical localization of acid phosphatase activity in the digestive caeca of the desert locust.Jl R. microsc. Soc. 88 279–89.
Burstone, M. s. (1958). Histochemical demonstration of acid phosphatase using naphthol AS phosphates.J. natn. Cancer Inst. 21, 523–39.
Cameron, L. (1912). Structure of the alimentary canal ofBacillus rossii (plasmidae).Proc. Zool. Soc. London,172, 69–77.
Forbes, A. r. (1964). The morphology, histology and fine structure of the gut of the green peach aphid,Myzus persicae (Sulzer).Mem. ent. Soc. 36, 1–74.
Gahan, P. b., Mclean, J., Kalina, M. &Sharma, W. (1967). Freeze-sectioning of plant tissues; the technique and its use in plant histochemistry.J. exp. Bot. 18, 151–9.
Gahan, P. b. &Mclean, J. (1967). Acid phosphatases in roots ofVicia faba.Biochem. J. 102, 47–48P.
Glauert, A. m. (1965). InTechniques for Electron Microscopy (ed. D. H. Kay). Oxford: Blackwell Scientific Publ. Ltd.
Holt, S. j. (1959). Factors governing the validity of staining methods for enzymes and their bearing upon the Gomori acid phosphatase technique.Expl Cell Res. Suppl.7, 1–27.
Holt, S. j. &Hicks, R. m. (1961). Localization of acid phosphatases in rat liver cells as revealed by combined cytochemical staining and electron microscopy.J. biophys. biochem. Cytol. 11, 47–66.
Maggi, V., Franks, L. m. &Carbonell, A. w. (1966). Histochemical study of a number of hydrolases, including a new acid phosphatase, in various tissues of men and mice.Histochemie 6, 305–16.
neil, n. w. & horner, m. w. (1962). The acid p-nitrophenyl phosphatase activity of adult guinea pig liver subcellular fractions.Biochem. J. 84, 32P.
Nelson, D. (1966). Rat liver acid phosphatases. Differences in lysosomal and cytoplasmic forms.Proc. Soc. exp. Biol. N.Y. 121, 998–1001.
Niles, N. r., Chayen, J., Cunningham, G. j. &Bitensky, L. (1965). The histochemical demonstration of adenosine triphosphatase activity in myocardium.J. Histochem. Cytochem. 12, 740–3.
Novikoff, A. b. (1963). Lysosomes in the physiology and pathology of cells: contributions of staining methods. InLysosomes, CIBA Found. Symp. London: Churchill.
Ornstein, L. (1964). Disc electrophoresis.Ann. N.Y. Acad. Sci. 121, 321–79.
Ramsay, J. a. (1955). The excretory system of the stick insect.J. exp. Biol. 32, 183–99.
Rosenbaum, R. m. &Rolon, C. i. (1962). Variability and specificity of intracellular acid phosphatases.Histochemie 3, 1–16.
Wigglesworth, V. b. &Salpeter, M. m. (1962). histology of malpighian tubules inRhodnius prolixus.J. Insect Physiol. 8, 299–307.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beadle, D.J., Gahan, P.B. Cytochemical studies of the types and localization of acid phosphatases in the various regions of the midgut epithelium ofCarausius morosus . Histochem J 1, 539–549 (1969). https://doi.org/10.1007/BF01012860
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01012860