Synopsis
A histochemical study of alkaline phosphatase in heterolysosomes (demonstrated by their content of acid phosphatase and β-glucuronidase) is described in the endocytizing epithelia of the neonate rat small intestine. The possible origins and functional significance of the enzyme in these organelles is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, W. A. (1968). Cytochemistry of sea urchin gametes. III. Acid and alkaline phosphatase activity of spermatozoa and fertilization.J. Ultrastruct. Res. 25, 1–14.
Baggiolini, M., Hirsch, J. G. &de Duve, C. (1969). Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation,J. Cell. Biol. 40, 529–41.
Barka, T. &Anderson, P. j. (1965).Histochemistry. Theory, practice and bibliography. New York: Hoeber.
Clark, S. l. (1961). Alkaline phosphatase of the small intestine studied with the electron microscope in suckling and adult mice.Anat. Rec. 139, 216.
Coffey, J. w. &de Duve, C. (1968). Digestive activity of lysosomes. I. The digestion of proteins by extracts of rat liver lysosomes.J. biol. Chem. 243, 3255–63.
Cohn, Z. a. &Hirsch, J. g. (1960). The isolation and properties of specific cytoplasmic organelles of rabbit polymorphonuclear leukocytes.J. exp. Med. 112, 983–1004.
de Duve, C. &Wattiaux, R. (1966). Functions of lysosomes.A. Rev. Physiol. 28, 435–92.
dingle, j. t. & dott, h. m. (1969). Lysosomal enzymes in bull and ram sperm,Biochem. J. III, 35p.
Fishman, W. h. &Goldman, S. s. (1965). A post-coupling technique for β-glucuronidase employing the substrate naphthol-AS-BI-β-D-glucosiduronic acid.J. Histochem. Cytochem. 13, 441–7.
Gomori, G. (1952).Microscopic Histochemistry, Chicago: Univ. Chicago Press.
harrison, r. a. p. & white, i. g. (1969). Distribution and behaviour of some glycolytic enzymes in spermatozoa and cytoplasmic droplets from mammalian semen.Biochem. J. 111, 36p.
Hayashi, M. (1967). Comparative histochemical localization of lysosomal enzymes in rat tissues.J. Histochem. Cytochem. 15, 83–92.
Hayashi, M., Nakajima, Y. &Fishman, W. h. (1963). The cytological demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin. A preliminary report.J. Histochem. Cytochem. 12, 293–7.
Heringová, A., Jirsová, V. &Koldovsky, O., (1965). Postnatal development of β-glucuronidase in the jejunum and ileum of rats.Can. J. Biochem. 43, 173–8.
Holt, J. h. &Miller, D. (1962). The localization of phosphomonoesterase and aminopeptidase in brush borders isolated from intestinal epithelium cells.Biochim. biophys. Acta 58, 239–43.
Hsu, L. &Tappel, A. l. (1964). Lysosomal enzymes in rat intestinal mucosa.J. Cell Biol. 23, 233–40.
Hübscher, G., West, G. r. &Brindley, D. n. (1965). Studies on the fractionation of mucosal homogenates from the small intestine.Biochem. J. 97, 629–42.
Hugon, J. &Borgers, M. (1966). Ultrastructural localization of alkaline phosphatase activity on the absorbing cells of the duodenum of the mouse.J. Histochem. Cytochem. 14, 629–40.
Hugon, J. &Borgers, M. (1968a). Fine structural localization of acid and alkaline phosphatase activities in the absorbing cells of the duodenum of rodents.Histochemie 21, 42–66.
Hugon, J. &Borgers, M. (1968b). Fine structural localization of three lysosomal enzymes and non-specific alkaline phosphatase in the villus of the human duodenum.Gastroenterol. 55, 608–18.
Koldovský, O., Faltová, O. e., Hahn, P. &Vacek, Z. (1960). InThe Development of Homeostasis. Liblice (1960). Symposium Czechoslovak Academy of Sciences. London & New York: Academic Press (1962).
Novikoff, A., Essner, E. &Quintana, N. (1962). Lysosomes.Fedn Proc. 23, 1010–22.
Vacek, Z. (1964). Submikroskopická struktura a cytochemie epitelu tenkého stréva u krysich mlád'atn.Čslká. Morf. 12, 292–301.
Wetzel, B. k., Spicer, S. s. &Horn, R. g. (1963). Cytochemical localization of non specific phosphatase activity in rabbit myeloid elements.J. Histochem. Cytochem,11, 812–14.
Wetzel, B. k., Spicer, S. s. &Horn, R. g. (1967). Fine structural localization of acid and alkaline phosphatases in cells of rabbit myeloid elements.J. Histochem. Cytochem. 15, 311–34.
Williams, R. m. &Beck, F. (1968). Intracellular association of pinocytotic capacity and lysosomal enzyme activity in the mammalian small intestine.J. Anat. 104, 175.
Yonge, C. m. (1937). Evolution and adaptation in the digestive system of the metazoa.Biol. Rev. 12, 87–115.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Williams, R.M., Beck, F. Demonstration of alkaline phosphatase in the lysosomes of neonate rat ileum. Histochem J 1, 531–538 (1969). https://doi.org/10.1007/BF01012859
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01012859