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Alkaline phosphatase and myoepithelial cells in the parotid gland of the rat

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Alkaline phosphatase activity has been studied in the parotid glands of rats at the light and electron microscopical levels. Reaction product was found to outline the plasma membranes of myoepithelial cells. It was also found in the walls of many capillaries and on the luminal surface and between apposing cells in some intercallary ducts.

The distribution of myoepithelial cells in the rat parotid is unusual. The cells run longitudinally around intercalary ducts and send processes on to the bases of adjoining acini but do not embrace the acini. The possible functions of these myoepithelial cells are discussed.

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References

  • Archer, F. L. &Kao, V. C. Y. (1968). Immunohistochemical identification of actomyosin in myoepithelium of human tissue.Lab. Invest. 18, 669–74.

    Google Scholar 

  • Bogart, B. I. (1968). The fine structural localization of alkaline phosphatase and acid phosphatase activity in the rat submandibular gland.J. Histochem. Cytochem. 16, 572–81.

    Google Scholar 

  • Bogart, B. I. (1971). Fine-structural localization of acetylcholinesterase activity in rat parotid and sublingual glands.Am. J. Anat. 132, 259–66.

    Google Scholar 

  • Bohman, S. O. &Maunsbach, A. B. (1970). Effects on tissue fine structure of variations in colloid osmotic pressure of glutaraldehyde fixatives.J. Ultrastruct. Res. 30, 195–208.

    Google Scholar 

  • Cartensen, E. L., Aldridge, W. G., Child, S. Z., Sullivan, P. &Brown, H. H. (1971). Stability of cells fixed with glutaraldehyde and acrolein.J. Cell Biol. 50, 529–32.

    Google Scholar 

  • Davies, K.J. &Garrett, J. R. (1972). Improved preservation of alkaline phosphatase in salivary glands of the cat.Histochem. J. 4, 365–79.

    Google Scholar 

  • Dewey, M. M. (1958). A histochemical and biochemical study of the parotid gland in normal and hypophysectomized rats.Am. J. Anat. 102 243–71.

    Google Scholar 

  • Emmelin, N., Garrett, J. R. &Ohlin, P. (1968). Neural control of salivary myoepithelial cells.J. Physiol. 196, 381–96.

    Google Scholar 

  • Emmelin, N., Garrett, J. R. &Ohlin, P. (1969). Motor nerves of salivary myoepithelial cells in dogs.J. Physiol. 200, 539–46.

    Google Scholar 

  • Emmelin, N., Garrett, J. R. &Ohlin, P. (1970). Actions of kinins on salivary myoepithelial cells.J. Physiol. 207, 539–44.

    Google Scholar 

  • Garrett, J. R. &Harrison, J. D. (1970). Alkaline-phosphatase and adenosine-triphosphatase histochemical reactions in the salivary glands of cat, dog and man, with particular reference to the myoepithelial cells.Histochemie 24, 214–29.

    Google Scholar 

  • Halpern, B. N. (1956). Histamine release by long chain molecules. In:Ciba Foundation Symposium on Histamine (eds. G. E. W. Wolstenholme and C. O. O'Connor), pp. 92–123. London: Churchill.

    Google Scholar 

  • Harrop, T. J. &Mackay, B. (1968). Electronmicroscopic observations on myoepithelial cells and secretory nerves in rat salivary glands.J. Canad. dent. Assoc. 34, 480–8.

    Google Scholar 

  • Hugon, J. &Borgers, M. (1966a) A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.J. Histochem. Cytochem. 14, 429–31.

    Google Scholar 

  • Hugon, J. &Borgers, M. (1966b). Ultrastructural localization of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse.J. Histochem. Cytochem. 14, 629–40.

    Google Scholar 

  • Karnovsky, M. J. (1965). A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy.J. Cell Biol. 27, 137–8A.

    Google Scholar 

  • Leeson, T. S. &Leeson, C. R. (1971). Myoepithelial cells in the exorbital lacrimal and parotid glands of the rat in frozen-etched replicas.Am. J. Anat. 132, 133–46.

    Google Scholar 

  • Novikoff, A. B. (1962). Cytochemical staining methods for enzyme activities; their application to the rat parotid gland.Jewish mem. Hosp. Bull. 7, 70–93.

    Google Scholar 

  • Parks, H. F. (1961). On the fine structure of the parotid gland of mouse and rat.Am. J. Anat. 108, 303–29.

    Google Scholar 

  • Scott, B. N. &Pease, D. C. (1959). Electron microscopy of the salivary and lacrimal glands of the rat.Am. J. Anat. 104, 115–61.

    Google Scholar 

  • Shear, M. (1966). The structure and function of myoepithelial cells in salivary glands.Archs oral Biol. II, 769–80.

    Google Scholar 

  • Shear, M. (1968). Histological and histochemical studies of intercalated ducts in rat parotid glands.S. Afr. J. med. Sci. 33, 97–103.

    Google Scholar 

  • Stutte, H. J. (1967). Hexazotiertes Triamino-tritolyl-methanchlorid (Neufuchsin) als Kupplungs-salz in der Ferment histochemie.Histochemie 8, 327–31.

    Google Scholar 

  • Sugimoto, A. (1960). Histochemical observations of the rat salivary glands.Proc. Jap. Histochem. Soc. 1, 152–61.

    Google Scholar 

  • Tamarin, A. (1966). Myoepithelium of the rat submaxillary gland.J. Ulstrastruct. Res. 16, 320–38.

    Google Scholar 

  • Tandler, B. (1965). Ultrastructure of the human submaxillary gland. III Myoepithelium.Z. Zellforsch. mikrosk. Anat. 65, 852–63.

    Google Scholar 

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Garrett, J.R., Parsons, P.A. Alkaline phosphatase and myoepithelial cells in the parotid gland of the rat. Histochem J 5, 463–471 (1973). https://doi.org/10.1007/BF01012003

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  • DOI: https://doi.org/10.1007/BF01012003

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