Skip to main content
SpringerLink
Log in

Myocardial cell heterogeneity in the human heart with respect to myosin ATPase activity

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The pH sensitivity of the Ca2+-activated myosin ATPase in atrial, ventricular and conduction tissue of human hearts has been established. Heterogeneity with respect to ATPase activity is shown not only to exist between the atrial, the ventricular myocardium and the conduction system but alsowithin both the ordinary atrial and ventricular myocardium andwithin the conduction system. These observations are related to the polymorphism of the myosin molecule and suggest that fibre types with different contractile properties co-exist in the human heart.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barany, M. (1967) ATPase activity of myosin correlated with speed of muscle shortening.J. gen. physiol. 50, 197–218.

    Google Scholar 

  • Billeter, R., Weber, H., Lutz, H., Howald, H., Eppenberger, H. M. &Jenny, E. (1980) Myosin types in human skeletal muscle fibers.Histochemistry 65, 249–59.

    Google Scholar 

  • Brooke, M. H. &Kaiser, K. K. (1970) Muscle fiber type: How many and what kind?Archs Neurol. 23, 369–79.

    Google Scholar 

  • Carey, R. A., Bove, A. A., Coulson, R. L., Packman, D. L. &Spann, J. F. (1979) Correlation between cardiac muscle myosin ATPase activity and velocity of muscle shortening.Biochem. Med. 21, 235–45.

    Google Scholar 

  • Cummins, P., Price, K. M. &Littler, W. A. (1980) Foetal myosin light chain in human ventricle.J. Muscle Res. Cell Motility 1, 357–66.

    Google Scholar 

  • Dalla Libera, L., Sartore, S. &Schiaffino, S. (1979) Comparative analysis of chicken atrial and ventricular myosins.Biochim. biophys. Acta 581, 283–94.

    Google Scholar 

  • Dubowitz, V. &Brooke, M. H. (1973) Histological and histochemical stains and reactions. InMuscle Biopsy (edited byDubowitz, V. andBrooke, M. H.), pp. 20–33. Philadelphia: Saunders.

    Google Scholar 

  • Elias, E. A., De Vries, G. P., Elias, R. A., Tigges, A. J. &Meijer, A. E. F. H. (1980) Enzyme histochemical studies on the conducting system of the human heart.Histochem. J. 12, 577–89.

    Google Scholar 

  • Eriksson, A., Thornell, L. E. &Stigbrand, T. (1979) Skeletin immunoreactivity in heart Purkinje fibers from several species.J. Histochem. Cytochem. 27, 1604–9.

    Google Scholar 

  • Flink, I. L., Rader, J. H., Banerjee, S. K. &Morkin, E. (1978) Atrial and ventricular cardiac myosins contain different heavy chain species.FEBS Lett. 94, 125–30.

    Google Scholar 

  • Gauthier, G. F. &Lowey, S. (1979) Distribution of myosin isoenzymes among skeletal muscle fibre types.J. Cell Biol. 81, 10–25.

    Google Scholar 

  • Guth, L. (1973) Fact and artifact in the histochemical procedure for myofibrillar ATPase.Expl Neurol. 41, 440–50.

    Google Scholar 

  • Guth, L. &Samaha, F. J. (1969) Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle.Expl Neurol. 25, 138–52.

    Google Scholar 

  • Guth, L. &Samaha, F. J. (1970) Procedure of the histochemical demonstration of actomyosin ATPase.Expl Neurol. 28, 365–7.

    Google Scholar 

  • Hoh, J. F. Y., McGrath, P. A. &Hale, P. T. (1978) Electrophoretic analysis of multiple forms of rat cardiac myosin: Effects of hypophysectomy and thyroxine replacement.J. molec. cell. Cardiol. 10, 1053–76.

    Google Scholar 

  • Klotz, C., Aumont, M. C., Leger, J. J. &Swynghedauw, B. (1975) Human cardiac myosin ATPase and light subunits a comparative study.Biochim. biophys. Acta 386, 461–9.

    Google Scholar 

  • Long, L., Fabian, F., Mason, D. T. &Wikman-Coeffelt, J. (1977). A new cardiac myosin characterized from the canine atria.Biochem. biophys. Res. Commun. 76, 626–35.

    Google Scholar 

  • Lutz, H., Ermine, M., Jenny, E., Bruggmann, S., Joris, F. &Weber, E. (1978) The size of the fiber populations in rabbit skeletal muscles as revealed by indirect immunofluorescence with anti-myosin sera.Histochemistry 57, 223–35.

    Google Scholar 

  • Lutz, H., Weber, H., Billeter, R. &Jenny, E. (1979) Fast and slow myosin within single skeletal muscle fibres of adult rabbits.Nature, Lond. 281, 142–4.

    Google Scholar 

  • Mabuchi, K. &Sreter, F. A. (1980) Actomyosin ATPase. I. Quantitative measurement of activity in cryostat sections.Muscle Nerve 3, 227–32.

    Google Scholar 

  • McFarland, B. H. &Inesi, G. (1971) Solubilization of sarcoplasmic reticulum with Triton X-100.Archs Biochem. Biophys. 145, 456–64.

    Google Scholar 

  • Meijer, A. E. F. H. (1970) Histochemical method for the demonstration of myosin adenosine triphosphatase in muscle tissues.Histochemie 22, 51–8.

    Google Scholar 

  • Morales, A. R. M. D. &Fine, G. M. D. (1965) Enzyme histochemistry of the sinus and atrioventricular nodes of the human heart.Lab. Invest. 14, 321–9.

    Google Scholar 

  • Morkin, E., Banerjee, S. K. &Stern, L. Z. (1977) Biochemical and histochemical evidence for stimulation of myosin ATPase activity in thyrotoxic rabbit heart.FEBS Lett. 79, 357–60.

    Google Scholar 

  • Price, K. M., Littler, W. A. &Cummins, P. (1980a) Myosin adenosine-triphosphatase activity and light chain subunit composition of human right and left ventricle.Cardiovas. Res. 14, 555–60.

    Google Scholar 

  • Price, K. M., Littler, W. A. &Cummins, P. (1980b) Human atrial and ventricular myosin light chain subunits in the adult and during development.Biochem. J. 191, 571–80.

    Google Scholar 

  • Samaha, F. J. &Yunis, E. J. (1973) Quantitative and histochemical demonstration of a calcium activated mitochondrial ATPase in skeletal muscle.Expl Neurol. 41, 431–9.

    Google Scholar 

  • Sartore, S., Pierobon-Bormioli, S. &Schiaffino, S. (1978) Immunohistochemical evidence for myosin polymorphism in the chicken heart.Nature, Lond. 274, 82–3.

    Google Scholar 

  • Sartore, S., Gorza, L., Pierobon-Bormioli, S., Dalla Libera, L. &Schiaffino, S. (1981) Myosin types and fiber types in cardiac muscle. I. Ventricular myocardium.J. Cell Biol. 88, 226–33.

    Google Scholar 

  • Scheuer, J. &Bhan, A. K. (1979) Cardiac contractile proteins. Adenosine triphosphatase activity and physiological function.Circ. Res. 45, 1–12.

    Google Scholar 

  • Schiaffino, S. &Pierobon-Bormioli, S. (1973) Histochemical characterization of adenosine triphosphatases in skeletal muscle fibers by selective extraction procedures.J. Histochem. Cytochem. 21, 142–5.

    Google Scholar 

  • Schiaffino, S., Gorza, L., Pierobon-Bormioli, S. &Sartore, S. (1980) Myosin polymorphism cellular heterogeneity and plasticity of cardiac muscle. InPlasticity of Muscle (edited byPette, D.), pp. 559–568. Berlin, New York: Walter de Gryter.

    Google Scholar 

  • Swynghedauw, B., Meignan, M., Piquet, V. &Hatt, P. Y. (1971) L'ATPase myofibrillaire myocardique dans l'insuffisance cardiaque.Arch. Mal. C. 65, 1547–56.

    Google Scholar 

  • Syrovy, I., Delcayre, C. &Swynghedauw, B. (1979) Comparison of ATPase activity and light subunits in myosins from left and right ventricles and atria in seven mammalian species.J. molec. cell. Cardiol. 11, 1129–35.

    Google Scholar 

  • Talesara, G. L. &Goldspink, G. (1978) Histochemical and biochemical study of myofibrillar ATPase in cardiac muscle of several species of bird.Histochem. J. 10, 695–710.

    Google Scholar 

  • Thornell, L.-E., Eriksson, A., Stigbrand, T. &Sjöström, M. (1978) Structural proteins in cow Purkinje and ordinary ventricular fibres. A marked difference.J. molec. cell Cardiol. 10, 605–16.

    Google Scholar 

  • Thornell, L.-E. &Eriksson, A. (1981) Filament systems in the Purkinje fibres of the heart.Am. J. Physiol. 241 (Heart Circ. Physiol. 10) H291-H305.

    Google Scholar 

  • Urthaler, F., Walker, A. A., Hefner, L. L. &James, T. N. (1975) Comparison of contractile performance of canine atrial and ventricular muscles.Circ. Res. 37, 762–71.

    Google Scholar 

  • Urthaler, F., Walker, A. A., Kawamura, K., Hefner, L. L. &James, T. N. (1978) Canine atrial and ventricular muscle mechanics studied as a function of age.Circ. Res. 42, 703–13.

    Google Scholar 

  • Whalen, R., Thornell, L.-E. &Eriksson, A. (1980) Heart Purkinje fibers contain both atrial—embryonic and ventricular—type myosin light chains.Eur. J. Cell. Biol. 22, 319.

    Google Scholar 

  • Whalen, R. G., Sell, S. M., Eriksson, A. & Thornell, L.-E. (1981) Myosin subunit types in skeletal and cardiac tissues and their developmental distribution.Devl Biol. (in press).

  • Wikman-Coeffelt, J. &Srivastava, S. (1979) Differences in atrial and ventricular myosin light chain LC1.FEBS Lett. 106, 207–12.

    Google Scholar 

  • Yazaki, Y., Ueda, S., Nagai, R. &Shimada, K. (1979) Cardiac atrial myosin adenosine triphosphatase of animals and humans. Distinctive enzymatic properties compared with cardiac ventricular myosin.Circ. Res. 45, 522–7.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden

    L. -E. Thornell & S. Forsgren

Authors
  1. L. -E. Thornell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Forsgren
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thornell, L.E., Forsgren, S. Myocardial cell heterogeneity in the human heart with respect to myosin ATPase activity. Histochem J 14, 479–490 (1982). https://doi.org/10.1007/BF01011858

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01011858

Keywords

Search

Navigation