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Differences of adenylate cyclase localization in guinea-pig peritoneal macrophages under different physiological conditions: An ultracytochemical study

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Summary

The ultrastructural localization of adenylate cyclase activity has been investigated in unfixed guinea-pig peritoneal macrophages in different physiological states (such as suspension, adhesion and phagocytosis) using a medium containing 5′-adenylyl-imidodiphosphate (AMP-PNP) as the substrate.

Adenylate cyclase activity was observed in cytoplasmic vacuoles of macrophages in suspension; in the perinuclear space, endoplasmic reticulum, Golgi complex and pseudopods of adherent macrophages; and surrounding phagocytosed polystyrene particles. The activity was inhibited by Alloxan added to the incubation medium and no staining was observed when AMP-PNP was omitted from the medium.

The segregation of this enzyme to phagocytic vacuoles and pseudopods may have significant implications in understanding cyclic nucleotide function in adhesion and phagocytosis.

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Authors and Affiliations

  1. Institute of General Pathology of Florence University, Viale Morgagni 50, 50134, Florence, Italy

    Germana Dini & Mario Del Rosso

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  1. Germana Dini
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  2. Mario Del Rosso
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Dini, G., Del Rosso, M. Differences of adenylate cyclase localization in guinea-pig peritoneal macrophages under different physiological conditions: An ultracytochemical study. Histochem J 15, 911–918 (1983). https://doi.org/10.1007/BF01011829

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  • DOI: https://doi.org/10.1007/BF01011829

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