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Visualization of retinal and choroidal blood flow with fluorescein leukocyte angiography in rabbits

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Abstract

• Purpose: To visualize the retinal and choroidal leukocytes in rabbits with a new technique, fluorescein leukocyte angiography using a scanning laser ophthalmoscope. • Methods: Blood was withdrawn from an ear vein of a rabbit (New Zealand White), mixed with fluorescein dye in a test tube and centrifuged. The yellow-brown coat layer containing fluorescein-stained leukocytes was collected and injected into the ear vein of the same rabbit while performing fluorescein angiography with a scanning laser ophthalmoscope. The angiographic image displaying circulating fluorescent leukocytes in retinal and choroidal vessels was recorded on a videotape. • Results: Fluorescent leukocytes were clearly visible in the retinal arteries, capillaries, veins and choroidal vessels for more than 1 h. Plugging of leukocytes was seen throughout this period of time in choroidal vessels, while plugging was rare in retinal vessels. • Conclusions: Fluorescein leukocyte angiography is a new technique which can be used for visualization of the leukocytes in retinal and choroidal vessels non-invasively and in vivo.

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Authors and Affiliations

  1. Department of Ophthalmology, School of Medicine, Wonkwang University, 344-2 Sinyoung-dong, 570-180, Iksan, Chonbuk, South Korea

    Yunsik Yang, Sangduck Kim & Jaeduck Kim

Authors
  1. Yunsik Yang
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  2. Sangduck Kim
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  3. Jaeduck Kim
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Yang, Y., Kim, S. & Kim, J. Visualization of retinal and choroidal blood flow with fluorescein leukocyte angiography in rabbits. Graefe's Arch Clin Exp Ophthalmol 235, 27–31 (1997). https://doi.org/10.1007/BF01007834

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  • DOI: https://doi.org/10.1007/BF01007834

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