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Endochitinase and β-1,3-glucanase genes are developmentally regulated during somatic embryogenesis inPicea glauca

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Abstract

Two cDNAs isolated from white spruce [Picea glauca (Moench) Voss] somatic embryos, are predicted to encode a basic class IV chitinase and a β-1,3-glucanase, respectively corresponding to genesPgChi-1 andPgGlu-1. Each represents a multigene family in spruce. Transcripts homologous toPgChi-1 orPgGlu-1 genes were highly abundant in embryogenic tissues and gradually decreased after tissues were placed on abscisic acid-containing maturation medium, with lowest abundance in globular embryos. Transcripts related toPgGlu-1 became highly abundant again in early cotyledonary embryos but decreased thereafter, whereas transcripts related toPgChi-1 were also highly abundant in late cotyledonary embryos and plantlets in vitro; transcripts were either low (PgChi-1) or were not detectable (PgGlu-1) in needles. Wounding, drying and flooding stresses enhancedPgChi-1-andPgGlu-1-related gene expression. Fungal cell wall suspension enhancedPgGlu-1-related transcript accumulation, but reducedPgChi-1-related transcript abundance within 24 h.PgChi-1 andPgGlu-1 and their homologues may have roles in plant defense, and possibly developmental roles during spruce somatic embryo maturation.

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Abbreviations

PR:

pathogenesis related

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Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada

    Jin-Zhuo Dong & David I. Dunstan

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  1. Jin-Zhuo Dong
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  2. David I. Dunstan
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Correspondence to David I. Dunstan.

Additional information

Nucleotide sequences appear in the GenBank Nucleotide Sequence Databases under the accession numbers L42467 (PgChi-1) and L49179 (PgGlu-1)

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Dong, JZ., Dunstan, D.I. Endochitinase and β-1,3-glucanase genes are developmentally regulated during somatic embryogenesis inPicea glauca . Planta 201, 189–194 (1997). https://doi.org/10.1007/BF01007703

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  • DOI: https://doi.org/10.1007/BF01007703

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