Synopsis
In this paper, model experiments on chicken red blood cell nuclei are described concerning the influence of methanol-acetic acid fixation and irradiation at different wavelengths, with and without prior Atebrin staining on subsequent Feulgen-stainability. In addition, data are reported on the influence on Feulgenstainability of Giemsa-banding procedures, illumination of unstained chromosomes at 220 and 515 nm and exposure of unstained and Atebrin-stained chromosomes to illumination at 440 nm.
The ASG and especially the trypsin-Giemsa technique appeared to reduce markedly Feulgen-stainability. The same holds true for Atebrin fluorescence of chromosomes. The data are discussed in relation to their implications for the assumed cause of the Q- and G-banding phenomena. Techniques are described that allow reliable Feulgen DNA measurements of individual chromosomes after application of either G- or Q-banding.
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Bosman, F.T., Van Der Ploeg, M. & Geraedts, J.P.M. Influence of Q- and G-banding on the Feflgen-stainability of human metaphase chromosomes. Histochem J 9, 31–42 (1977). https://doi.org/10.1007/BF01007007
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DOI: https://doi.org/10.1007/BF01007007