Skip to main content
SpringerLink
Log in

Pyridoxal 5′-phosphate: a possible physiological substrate for alkaline phosphatase in human neutrophils

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The intracellular localization of pyridoxal phosphatase activity was demonstrated in human neutrophils by electron microscope cytochemistry. Under alkaline conditions, an enzyme active against pyridoxal phosphate was localized to a cytoplasmic granule population, the phosphasome. These granules have previously been shown by electron microscope cytochemical techniques and by subcellular fractionation to be rich in alkaline phosphatase. Under acidic conditions, a phosphatase activity against pyridoxal phosphate was localized to intracellular multilamellar bodies resembling secondary lysosomes. These were quite distinct from the primary, secondary and phosphasome granules and this unique localization corresponds to that previously demonstrated (tertiary granules) by subcellular fractionation studies of these cells. The similarity in the enzyme reaction requirements of alkaline pyridoxal phosphatase and alkaline phosphatase, and their localization to the same subcellular organelle, suggests that pyridoxal phosphate may be a physiological substrate for human neutrophil alkaline phosphatase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bishayee, S. &Bachhawat, B. K. (1972) Subcellular distribution, age dependent variation and species differences of bran pyridoxal phosphate phosphatase.Neurobiology 2, 12–20.

    Google Scholar 

  • Ebadi, M. &Govitrapong, P. (1979) Microassay and properties of pyridoxal phosphate phosphatase in rat pineal gland.Int. J. Biochem. 10, 705–11.

    Google Scholar 

  • Ellis, J., Folkers, K., Levy, M., Takemura, K., Shizukuishi, S., Ulrich, R. &Harrison, P. (1981) Therapy with vitamin B6 with and without surgery for treatment of patients having idiopathic carpal tunnel syndrome.Res. Com. Chem. Path. Pharmacol. 33, 331–44.

    Google Scholar 

  • French, S. W. &Castagna, J. (1967) Some effects of chronic ethanol feeding on vitamin B6 deficiency in the rat.Lab Invest. 16, 526–31.

    Google Scholar 

  • Hines, J. D. (1976) Effect of pyridoxine plus chronic phlebotomy on the function and morphology of bone marrow and liver in pyridoxine-responsive sideroblastic anaemia.Sem. Hematol. 13, 133–40.

    Google Scholar 

  • Lumeng, L., Cleary, R. E., Wagner, R., Yu, P. L. &Li, T. K. (1976) Adequacy of vitamin B6 supplementation during pregnancy: a prospective study.Am. J. Clin. Nutr. 29, 1376–83.

    Google Scholar 

  • Lumeng, L., &Li, T. K. (1975) Characterization of the pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate hydrolase in rat liver.J. Biol. Chem. 250, 8126–31.

    Google Scholar 

  • Raja, K. B., Smith, G. P. &Peters, T. J. (1981) Subcellular localization and properties of alkaline inorganic pyrophosphatase in human polymorphonuclear leucocytes.Clin. Chim. Acta 117, 33–41.

    Google Scholar 

  • Rinehart, J. F. &Greenberg, L. D. (1949) Arteriosclerotic lesions in pyridoxine-deficient monkeys.Am. J. Path. 25, 481–91.

    Google Scholar 

  • Rustin, G. J. S. &Peters, T. J. (1979) Studies on the subcellular organelles of neutrophils in chronic granulocytic leukaemia with special reference to alkaline phosphatase.Br. J. Haematol. 41, 533–43.

    Google Scholar 

  • Rustin, G. J. S., Wilson, P. D. &Peters, T. J. (1979) Studies on the subcellular localisation of human neutrophil alkaline phosphatase.J. Cell Sci. 36, 401–12.

    Google Scholar 

  • Smith, G. P. &Peters, T. J. (1980a) Subcellular localisation and properties of adenosine triphosphatase in human polymorphonuclear leukocytes.Eur. J. Clin. Invest. 10, 475–80.

    Google Scholar 

  • Smith, G. P. &Peters, T. J. (1980b) Studies on the activities, kinetic properties and subcellular localisation of cAMP phosphodiesterase in human neutrophil leukocytes.Clin. Chim. Acta 103, 193–201.

    Google Scholar 

  • Smith, G. P. &Peters, T. J. (1981a) Assay and subcellular localisation of adenosine diphosphatase in human neutrophil polymorphonuclear leukocytes.Biochim. Biophys. Acta 673, 234–42.

    Google Scholar 

  • Smith, G. P. &Peters, T. J. (1981b) Subcellular localisation and properties of histone phosphate phosphatase in human polymorphonuclear leukocytes: alterations in pregnancy and chronic granulocytic leukaemia and relationship to alkaline phosphatase.Clin. Chim. Acta 111, 17–25.

    Google Scholar 

  • Smith, G. P. &Peters, T. J. (1981c) Subcellular localisation and properties of pyridoxal phosphate phosphatases of human polymorphonuclear leukocytes and their relationship to acid and alkaline phosphatase.Biochim. Biophys. Acta 661, 287–94.

    Google Scholar 

  • Smith, G. P., Smith, G. D. &Peters, T. J. (1981) A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase.Clin. Chim. Acta 114, 257–62.

    Google Scholar 

  • Wachstein, M., Kellner, J. D. &Ortiz, J. M. (1960) Pyridoxal phosphate in plasma and leukocytes in patients with leukaemia and other diseases.Proc. Soc. Exp. Biol. Med. 105, 563–6.

    Google Scholar 

  • Wilson, P. D., Rustin, G. J. S. &Peters, T. J. (1981) The ultrastructural localization of human neutrophil alkaline phosphatase in normal individuals during pregnancy and in patients with chronic granulocytic leukaemia.Histochem. J. 13, 31–43.

    Google Scholar 

Download references

Author information

Author notes

  1. P. D. Wilson

    Present address: Department of Medicine, University of Colorado Health Sciences Centre, 80262, Denver, Colorado, USA

Authors and Affiliations

  1. Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK

    P. D. Wilson, G. P. Smith & T. J. Peters

Authors
  1. P. D. Wilson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. P. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. J. Peters
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wilson, P.D., Smith, G.P. & Peters, T.J. Pyridoxal 5′-phosphate: a possible physiological substrate for alkaline phosphatase in human neutrophils. Histochem J 15, 257–264 (1983). https://doi.org/10.1007/BF01006240

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01006240

Keywords

Search

Navigation