Summary
The fine localization of acid phosphatase activity in yolk droplets in the notochordal cells of the developing chick (stage 12–13) has been investigated by electron microscopy. The enzyme reaction products are mainly found on the peripheries of yolk droplets of various different sizes, which are often clustered together to form larger masses. The rough endoplasmic reticulum and Golgi complexes with the substantial or small amounts of reaction product are closely, and occasionally directly, associated with the yolk droplets and their masses. These findings strongly suggested that both the rough and endoplasmic reticulum and Golgi complexes supply the acid phosphatase for the utilization of yolk in the differentiating notochordal cells.
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References
Bancroft, M. &Bellairs, R. (1976) The development of the notochord in the chick embryo, studied by scanning and transmission electron microscopy.J. Embryol. exp. Morph. 35, 383–401.
Barka, T. &Anderson, P. J. (1962) Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler.J. Histochem. Cytochem. 10, 741–53.
Cushman, S. W. (1970) Structure-function relationships in the adipose cell. I. Ultrastructure of the isolated adipose cell.J. Cell Biol. 46, 326–41.
Gomori, G. (1950) An improved histochemical technic for acid phosphatase.Stain Technol. 25, 81–5.
Hamburger, V. &Hamilton, H. L. (1951) A series of normal stage in the development of the chick embryo.J. Morph. 88, 49–92.
Jurand, A. (1962) The development of the notochord in chick embryos.J. Embryol. exp. Morph. 10, 602–21.
Jurand, A. &Selman, G. G. (1964) Yolk utilization in the notochord of the newt as studied by electron microscopy.J. Embryol. exp. Morph.,12, 43–50.
Kayahara, T. (1982) The fine localization of acid phosphatase activity in the unvacuolated notochordal cells of the early chick embryos.Histochem. J. 14, 347–60.
Lemanski, L. &Aldoroty, R. (1977) Role of acid phosphatase in the breakdown of yolk platelets in developing amphibian embryo.J. Morph. 153, 419–26.
Luft, J. H. (1961) Improvements in epoxy embedding method.J. Biophys. biochem. Cytol. 9, 409–14.
Waddington, C. H. &Perry, M. M. (1962) The ultrastructure of the developing urodele notochord.Proc. R. Soc. Lond. 156, 459–82.
Watson, M. (1958) Staining of tissue sections for electron microscopy with heavy metals.J. Biophys. biochem. Cytol. 4, 475.
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Kayahara, T. Acid phosphatase activity in yolk droplets of chick notochordal cells. Histochem J 15, 71–77 (1983). https://doi.org/10.1007/BF01006072
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DOI: https://doi.org/10.1007/BF01006072