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Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)

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Abstract

In this study, a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase, and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules (YGI, YGII, and YGIII) that differed in electron density and acid phosphatase reaction were identified in early cleavage, morula, blastula, gastrula, trochophore, and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation, indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI (free of acid phosphatase reaction) with YGII (rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered.

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Authors and Affiliations

  1. College of Life Science, Ludong University, Yantai, 264025, China

    Yiyan Wang  (王宜艳), Hushan Sun  (孙虎山), Yanjie Wang  (王艳杰), Dongchun Yan  (闫冬春) & Lei Wang  (王磊)

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  1. Yiyan Wang  (王宜艳)
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  2. Hushan Sun  (孙虎山)
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  3. Yanjie Wang  (王艳杰)
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  4. Dongchun Yan  (闫冬春)
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  5. Lei Wang  (王磊)
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Correspondence to Hushan Sun  (孙虎山).

Additional information

Supported by the Natural Science Foundation of Shandong Province (Nos. ZR2010CM017, ZR2012CM004) and the National Natural Science Foundation of China (No. 31101929)

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Wang, Y., Sun, H., Wang, Y. et al. Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg). Chin. J. Ocean. Limnol. 33, 339–346 (2015). https://doi.org/10.1007/s00343-015-3297-5

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  • DOI: https://doi.org/10.1007/s00343-015-3297-5

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