Synopsis
The surface coating of normal and abnormal trophoblast has been studied histochemically. It has been found to contain a highly sulphated mucosaccharide which is probably present together with an acidic non-sulphated mucosaccharide; there are suggestions that the accompanying protein is highly basic. The only difference noted between the coat on the normal trophoblast and that of the hydatidiform mole and ectopic trophoblast was the presence ofvis-glycol groups in the normal which were not found in the abnormal. It is suggested that the presence of the sulphated mucoprotein on the surface of the trophoblast is involved in the protection of the trophoblast from immunological attack.
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References
Boyd, J. D., Hamilton, W. H. &Boyd, C. A. R. (1968). The surface of the syncitium of the human chorionic villus.J. Anat. 102, 553–63.
Bradbury, S., Billington, W. D. &Kirby, D. R. S. (1965). A histochemical and electron microscopical study of the fibrinoid of the mouse placenta.Jl R. microsc. Soc. 84, 199–211.
Bradbury, S. &Stoward, P. J. (1967). The specific cytochemical demonstration in the electron microscope of periodate-reactive mucosubstances and polysaccharides containingvic-glycol.Histochemie 11, 71–80.
calatroni, A. &Di Ferrante, N. (1969). The glycosaminoglycans of human term placenta.Carbohyd. Res. 10, 535–48.
Currie, G. A. &Bagshawe, K. D. (1967). The masking of antigens on trophoblast and cancer cells.Lancet,1, 708–10
Currie, G. A., Van Dorninck, W. &Bagshawe, K. D. (1968). The effect of neuraminidase on the immunogenicity of early mouse trophoblast.Nature, Lond. 219, 191–2.
Gottschalk, A. (1957). Neuraminidase: The specific enzyme of influenza virus andVibrio cholerae.Biochim. biophys. Acta 23, 645.
Ito, S. (1965). The enteric surface coat on cat intestinal microvilli.J. Cell. Biol. 27, 475–91.
Kerr, M. G. (1968). Immunological rejection as a cause of abortion.J. Reprod. Fertil. Suppl. 3, 49.
Kirby, D. R. S., Billington, W. D., Bradbury, S. &Goldstein, D. J. (1964). The antigen barrier of the mouse placenta.Nature, Lond. 204, 548–9.
Matthews, M. B. (1967). Biophysical aspects of acid mucopolysaccharides relevant to connective tissue structure and function. InThe Connective Tissue (eds. B. W. Wagner and D. E. Smith), pp. 305–29. Baltimore: Williams & Wilkins.
Pearse, A. G. E. (1968).Histochemistry, Theoretical and Applied, Vol. 1. 3rd Ed. London: Churchill.
Pease, D. C. (1966). Polysaccharides associated with the exterior surface of epithelial cells: kidney, intestine, brain.J. Ultrastruct. Res. 15, 555–88.
Pratt, S. A. &Napolitano, L. (1969). Osmium binding to the surface coat of intestinal microvilli in the cat under various conditions.Anat. Rec. 165, 197–210.
Rambourg, A., Neutra, M. &Le Blond, C. P. (1966). Presence of a ‘cell coat’ rich in carbohydrate at the surface of cells in the rat.Anat. Rec. 154, 41–72.
Ravetto, C. (1964). Histochemical identification of sialic (neuraminic) acids.J. Histochem. Cytochem. 12, 306.
Revel, J. P. &Ito, S. (1965). The surface components of cells. InSpecificity of Cell Surfaces (eds. B. Davis and L. Warren), pp. 211–33. New York: Prentice-Hall.
Reynolds, E. S. (1963). The use of lead nitrate at high pH as an electron opaque stain in electron microscopy.J. Cell Biol. 17, 208–12.
Scott, J. E. &Dorling, J. (1965). Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions.Histochemie 5, 221–33.
Simmons, R. L. &Russell, P. S. (1962). The antigenicity of mouse trophoblast.Ann. N.Y. Acad. Sci. 99, 717–32.
Spicer, S. S. (19600. A correlative study of the histochemical properties of rodent acid mucopolysaccharides.J. Histochem. Cytochem. 8, 18–34.
Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967). Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (eds. B. W. Wagner and D. E. Smith) pp. 251–303. Baltimore: Williams & Wilkins.
Spicer, S. S., Leppi, T. J. &Stoward, P. J. (1965). Suggestions for a histochemical terminology of carbohydrate-rich tissue components.J. Histochem. Cytochem. 13, 599–603.
Spicer, S. S. &Lillie, R. D. (1961). Histochemical identification of basic proteins with Biebrich scarlet at alkaline pH.Stain Technol. 36, 365–70.
Stoward, P. J. (1967a). The histochemical properties of some periodate-reactive mucosubstances of the pregnant Syrian hamster before and after methylation with methanolic thionyl chloride.Jl R. microsc. Soc. 87, 77–103.
Stoward, P. J. (1967b). Histochemical studies of the formazan reaction. II. The conversion of periodatereactive mucosubstances into diphenyl- and phenyl-4′-diazo-3,3′-dimethoxybiphenylformazans and related derivatives.Jl R. microsc. Soc. 87, 407–35.
Tighe, J. R., Garrod, P. R. &Curran, R. C. (1967). The trophoblast of the human chorionic villus.J. Path. Bact. 93, 559.
Tock, E. P. C. &Pearse, A. G. E. (1965). Preservation of tissue mucins by freeze drying and vapour fixation. I. Light microscopy.Jl R. microsc. Soc. 84, 519–31.
Vickerman, K. (1969). On the surface coat and flagellar adhesion in trypanosomes.J. Cell. Sci. 5, 163–93.
Warren, L. (1959). The thiobarbituric acid assay of sialic acids.J. Biol. Chem. 234, 1971–5.
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Bradbury, S., Billington, W.D., Kirby, D.R.S. et al. Histochemical characterization of the surface mucoprotein of normal and abnormal human trophoblast. Histochem J 2, 263–274 (1970). https://doi.org/10.1007/BF01004995
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DOI: https://doi.org/10.1007/BF01004995