Synopsis
Alkaline phosphatase has been localized ultracytochemically in PMN of man with normal and elevated levels of this enzyme. Contrary to guinea-pig PMN, no activity appears to be present in the specific granules. Instead, the plasma membrane and the membrane of the endocytic vacuoles show a strong staining. However, the demonstration of this activity depends on the preparatory procedure employed for PMN isolation. the use of dextran and Ficoll-Hypaque in the isolation procedure induces a marked increase in alkaline phosphatase staining of the PMN plasma membrane. Strongly increased activity at this site has been found in PMN from cancer patients. In most of them, additional staining has been observed in atypical vesicles and sometimes in the Golgi apparatus. These findings are discussed in the light of some previously reported controversial biochemical and cytochemical data on the distribution of alkaline phosphatase in human PMN.
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References
Anderson, D. R. (1965). A method for preparing peripheral leukocytes for electron microscopy.J. Ultrastruct. Res. 13, 263–70.
Bainton, D. F. &Farquhar, M. G. (1968). Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. II. Cytochemistry and electron microscopy of bone marrow cells.J. Cell Biol. 39, 299–321.
Bainton, D. F., Ullyot, J. L. &Farquhar, M. G. (1971). The development of neutrophilic polymorphonuclear leukocytes in human bone marrow.J. exp. Med. 134, 907–34.
Borgers, M., Verheyen, A. & Thone, F. (1973). The inhibition of leukocyte alkaline phosphatase by new compounds.Abstr. Joint Session on Electron Microscopy. p. 65. Liège, Belgium.
Borgers, M. &Thone, F. (1975). The inhibition of alkaline phosphatase byl-p-bromotetramisole.Histochemistry 44, 277–80.
Boyum, A. (1968). Isolation of mononuclear cells and granulocytes from human blood, isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1g.Scand. J. clin. Lab. Invest. 21 (suppl. 97), 77–89.
Bretz, U. &Baggiolini, M. (1973). Association of the alkaline phosphatase of rabbit polymorphonuclear leukocytes with the membrane of the specific granules.J. Cell Biol. 59, 696–707.
Bretz, U. &Baggiolini, M. (1974). Biochemical and morphological characterization of azurophil and specific granules of human neutrophilic polymorphonuclear leukocytes.J. Cell Biol. 63, 251–69.
Briggs, R. T., Brath, D. B., Karnovsky, M. L. &Karnovsky, M. J. (1975). Localization of NADH-oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method.J. Cell Biol. 67, 566–86.
Fallon, H. J., Frei, E., Davidson, J. D., Trier, J. S., &Burk, D. (1962). Leukocyte preparations from human blood: evaluation of their morphologic and metabolic state.J. Lab. clin. Med. 59, 779–91.
Hugon, J. &Borgers, M. (1966). Ultrastructural localization of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse.J. Histochem. Cytochem. 14, 629–40.
Rufo, M. B. &Fishman, W. H. (1972).l-Homoarginine, a specific inhibitor of liver-type alkaline phosphatases, applied to the recognition of liver type enzyme activity in rat intestine.J. Histochem. Cytochem. 20, 336–43.
Spitznagel, J. K., Dalldorf, F. G., Leffell, M. S., Folds, J. D., Welsh, I. R. H., Cooney, M. H. &Martin, L. E. (1974). Character of azurophil and specific granules purified from human polymorphonuclear leukocytes.Lab. Invest. 30, 774–85.
Van Belle, H. (1976). Alkaline phosphatase. II. Conditions affecting determination of total activity in serum.Clin. Chemistry 22, 977–81.
West, B. C., Rosenthal, A. S., Gelb, N. A. &Kimball, H. R. (1974). Separation and characterization of human neutrophil granules.Am. J. Path. 77, 41–66.
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Borgers, M., Thoné, F., De Crée, J. et al. Alkaline phosphatase activity in human polymorphonuclear leukocytes. Histochem J 10, 31–43 (1978). https://doi.org/10.1007/BF01003412
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DOI: https://doi.org/10.1007/BF01003412