Skip to main content
SpringerLink
Log in

A preliminary note on the hormonal induction of NADP-linked dehydrogenases in phytohaemagglutinin-stimulated human lymphocytes

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

Human leucocytes were culturedin vitro with either phytohaemagglutin or 17 β-oestradiol or both, and the enzyme activities of nicotinamide adenine dinucleotide phosphate-linked glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase were assayed. As a result, it was found that these enzymes were considerably induced by oestradiol in phytohaemagglutinin-stimulated lymphocytes, but not in non-stimulated cells. Electrophoretic studies on glucose-6-phosphate dehydrogenase showed that six molecular forms are demonstrable in normal and phytohaemagglutinin-stimulated leucocytes, and that one of the forms was specifically induced by oestradiol in the presence of phytohaemagglutinin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bach, F. &Hirschhorn, K. (1963).r-globulin production by human lymphocytesin vitro.Expl Cell Res. 32, 592–5.

    Google Scholar 

  • Bain, B., Vaes, M. R. &Lowenstein, L. (1964). The development of large immature mononucleus cells in mixed leukocyte cultures.Blood 23, 108–16.

    Google Scholar 

  • Barker, K. L. &Warren, J. C. (1966). Template activity of uterine chromatin: control by estradiol.Proc. natn. Acad. Sci. U.S.A. 56, 1298–1302.

    Google Scholar 

  • Bender, M. A. &Prescott, D. M. (1962). DNA synthesis and mitosis in cultures of human peripheral leukocytes.Expl Cell Res. 27, 221–9.

    Google Scholar 

  • Byron, J. W. &Jajtha, L. G. (1962). Cellular kinetics of a peripheral blood cell system treated with phytohemagglutinin.Blood 20, 102.

    Google Scholar 

  • Carstairs, K. (1962). The human lymphocyte: its possible pluripotential quality.Lancet i, 829–33.

    Google Scholar 

  • Cooper, E. H., Barkhan, P. &Hale, A. J. (1963). Observations on the proliferation of human leukocytes cultured with phytohaemagglutinin.Brit. J. Haematol. 9, 101–11.

    Google Scholar 

  • Cooper, E. H. &Inman, D. R. (1964), Ultrastructure of human leucocytes synthesizing DNA.Nature, Lond. 204, 894.

    Google Scholar 

  • Cooperband, S. R., Green, J. A., Kennedy, M. A. &Grant, K. M. (1967). Dissociation and inhibition of the stimulating effect of phytohaemagglutinin on protein and DNA synthesis in human lymphocyte cultures.Nature, Lond. 214, 1240–1.

    Google Scholar 

  • Cowling, D. C. &Quaglino, D. (1965). Effect of some antigens on leucocyte cultures.J. Path. Bact. 89, 63–70.

    Google Scholar 

  • Davis, B. J. (1964). Disc electrophoresis—II. Method and application to human serum proteins.Ann. N.Y. Acad. Sci. 121, 404–27.

    Google Scholar 

  • Elrod, L. M. &Schrek, R. (1965). Production of lymphoblastoid cells from rat and human lympocytes by rabbit serum and by phytohemagglutinin.Expl Cell Res. 38, 418–23.

    Google Scholar 

  • Elves, M. E., Gouch, J., Chapman, J. A. &Israels, M. C. G. (1964). Electronmicroscopy studies of lymphocytes. Transformation under the influence of phytohaemagglutinin.Lancet i, 306–8.

    Google Scholar 

  • Elves, N. W. &Wilkinson, J. F. (1963). The effects of phytohaemagglutinin on normal and leukemic leukocyte when culturedin vitro.Expl Cell Res. 30, 200–7.

    Google Scholar 

  • Epstein, L. B. &Stohlman, F. Jr. (1964). RNA synthesis in cultures of normal human peripheral blood.Blood 24, 69–75.

    Google Scholar 

  • Forbes, I. J. (1965). Specific and non-specific stimulation of antibody synthesis by human leucocytein vitro.Lancet i, 198–9.

    Google Scholar 

  • Forsdyke, D. R. (1967). Quantitative nucleic acid changes during phytohaemagglutinin-induced lymphocyte transformationin vitro. Dependence of the response on phytohaemagglutinin/serum ratio.Biochem. J. 105, 679–84.

    Google Scholar 

  • DeFreitas, F. M., Faraco, E. Z., Deazevedo, D. F., Zaduchliver, J. &Lewin, I. (1965). Behaviour of normal pulmonary circulation during change of total blood volume in man.J. clin Invest. 44, 366–78.

    Google Scholar 

  • Glock, G. E. & McLean, P. (1953). Further studies on the properties and assay of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver.Biochem. J. 55, 400–8.

    Google Scholar 

  • Hale, A. J. &Cooper, E. H. (1965). Feulgen positive DNA in normal circulating lymphocytes, lower than in transformed, but non-DNA-synthesizing lymphocytes: In:Current Research in Leukemia (ed. F. G. J. Hayhoe). Cambridge: Cambridge University Press.

    Google Scholar 

  • Hayhoe, F. G. &Quaglino, D. (1965). Autographic investigation of RNA and DNA metabolism of human leucocytes cultured with phytohaemagglutinin; Uridine-5-3H as a specific precursor of RNA.Nature, Lond. 205, 151–4.

    Google Scholar 

  • Hirschhorn, K. F., Bach, F., Kolodny, R. L., Firschein, I. L. &Hashem, N. (1963). Immune response and mitosis of human peripheral blood lymphocytesin vitro.Science 142, 1185–7.

    Google Scholar 

  • Hori, S. H. &Kamada, T. (1967) Species difference in electrophoretically distinct forms of glucose 6-phosphate dehydrogenase in some mammals.Jap. J. Genet. 42, 367–74.

    Google Scholar 

  • Hori, S. H. &Matsui, S. (1967a). Effects of hormones on hepatic glucose 6-phosphate dehydrogenase of rat.J. Histochem. Cytochem. 15, 530–4.

    Google Scholar 

  • Hori, S. H. & Matsui, S. (1967b). Intracellular distribution of electrophoretically distinct forms of hepatic glucose 6-phosphate dehydrogenase.J. Histochem. Cytochem. (In press.)

  • Inman, D. R. &Cooper, E. H. (1963). Electronmicroscopy of human lymphocytes stimulated by phytohaemagglutinin.J. Cell Biol. 19, 441–5.

    Google Scholar 

  • Jackson, J. F. &Killander, D. (1964). DNA synthesis in phytohemagglutinin-stimulated human leukocyte cultures treated with β-mercaptoethanol.Expl Cell Res. 33, 459–67.

    Google Scholar 

  • Jensen, E. V. &Jacobson, H. I. (1962). Basic guides to the mechanism of estrogen action.Recent Prog. Horm. Res. 18, 387–414.

    Google Scholar 

  • Kay, J. E. (1967). Effect of phytohaemagglutinin on lymphocyte ribonucleic acid in synthesis.Biochem. J. 104, 66–7.

    Google Scholar 

  • Killander, D. &Rigler, R. Jr. (1965) Initial changes of deoxyribonucleoprotein and synthesis of nucleic acid in phytohemagglutinin-stimulated human leucocytesin vitro.Expl Cell Res. 39, 701–4.

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L. &Randall, R. J. (1951) Protein measurement with the Folin phenol reagent.J. biol Chem. 193, 265–75.

    Google Scholar 

  • Mackinney, A. A. Jr.,Stohlman, F. Jr. &Brecher, G. (1962). The kinetics of cell proliferation in cultures of human peripheral blood.Blood 19, 349–58.

    Google Scholar 

  • Marshall, W. H. &Roberts, K. B. (1963) The growth and mitosis of human small lymphocytes after incubation with a phytohemagglutinin.Q. Jl. exp. Physiol. 48, 146.

    Google Scholar 

  • Maurer, H. R. &Chalkley, G. R. (1967). Some properties of a nuclear binding site of estradiol.J. molec. Biol. 27, 431–41.

    Google Scholar 

  • McIntyre, O. R. &Ebaugh, F. G. Jr. (1962). The effect of phytohaemagglutinin on leukocyte cultures as measured by P18 incorporation in the DNA, RNA, and acid soluble fractions.Blood 19, 443–53.

    Google Scholar 

  • Michalowsky, A., Bartoszewicz, W. &Kozubowski, J. (1966). Blastic transformation of leukocyte cultures.Lancet ii, 1130–1.

    Google Scholar 

  • Mueller, G. C. &Mahieu, M. L. (1966). Induction of ribonucleic acid synthesis in human leukocytes by phytohaemagglutinin.Biochim. biophys. Acta 114, 100–7.

    Google Scholar 

  • Noteboom, W. D. &Gorski, J. (1965). Stereospecific binding of estrogen in the rat uterus.Archs. Biochem. Biophys. 111, 559–68.

    Google Scholar 

  • Ohno, S., Payne, H. W., Morrison, M. &Beutler, E. (1966). Glucose-6-phosphate dehydrogenase found in human liver.Science 153, 1015–16.

    Google Scholar 

  • Ornstein, L. (1964). Disc electrophoresis—I. Background and theory.Ann. N.Y. Acad. Sci. 121, 321–49.

    Google Scholar 

  • Parenti, F., Franceschini, P., Fortic, G. &Cepellini, R. (1966). The effect of phytohaemagglutinin on the metabolism and r-globulin synthesis of human lymphocytes.Biochim. biophys. Acta 123, 181–7.

    Google Scholar 

  • Parker, J. W., Wakasa, H. &Lukes, R. J. (1965). The morphological and cytochemical demonstration of lysosomes in lymphocytes incubated with phytohemagglutinin by electron microscopy.Lab. Invest. 14, 1736–43.

    Google Scholar 

  • Pearmain, G. E. &Lycette, R. R. (1963). Spontaneous mitosis of glandular-fever cells in short-term peripheral-blood cultures.Lancet ii, 1072.

    Google Scholar 

  • Pearmain, G. E., Lycette, R. R. &Fitzgerald, P. H. (1963). Tuberculin-induced mitosis in peripheral blood leucocytes.Lancet i, 637–8.

    Google Scholar 

  • Quaglino, D., Hayhoe, F. G. &Flemans, R. J. (1962). Cytochemical observations on the effect of phytohaemagglutinin in short term tissue cultures.Nature, Lond. 196, 338–40.

    Google Scholar 

  • Robbins, J. H. (1964). Tissue culture studies of the human lymphocyte. Experiments under controlled conditions provided new information on this cell's function and potentiality.Science 146, 1648–54.

    Google Scholar 

  • Rubin, A. D. &Cooper, H. L. (1965). Evolving patterns of RNA metabolism during transition from resting state to active growth in lymphocytes stimulated by photohemagglutinin.Proc. natn. Acad. Sci. U.S.A. 54, 469–76.

    Google Scholar 

  • Salzman, N. P., Pellegrino, P. &Fracheschini, P. (1966). Biochemical changes in PHA stimulated human lymphocytes.Expl Cell Res. 44, 75–83.

    Google Scholar 

  • Shaw, C. R. (1966). Glucose-6-phosphate dehydrogenase: Homologous molecules in deer mouse and man.Science 153, 1013–15.

    Google Scholar 

  • Shaw, C. R. &Kohen, A. L. (1963). Hormone-induced esterase in mouse kidney.Science 140, 70–1.

    Google Scholar 

  • Talwar, G. P., Segal, S. J., Evans, S. &Davidson, O. W. (1964). The binding of estradiol in the uterus: A mechanism for derepression of RNA synthesis.Proc. natn. Acad. Sci. U.S.A. 52, 1059–66.

    Google Scholar 

  • Tanaka, Y., Epstein, L. B., Brecher, G. &Stohlman, F. Jr. (1963). Transformation of lymphocytes in cultured of human peripheral blood.Blood 22, 614–29.

    Google Scholar 

  • Toft, D. &Gorski, J. (1966). A receptor molecule for estrogens: Isolation from the rat uterus and preliminary characterization.Proc. natn. Acad. Sci. U.S.A. 55, 1574–81.

    Google Scholar 

  • Torelli, U., Quaglino, D., Sauli, S. &Mauri, C. (1967). A cytochemical study on the DNA and histone changes occurring in phytohaemagglutinin stimulated lymphocytes.Expl Cell Res. 45, 218–8.

    Google Scholar 

  • Villee, G. A. (1955). An estradiol-induced stimulation of citrate utilization by placenta.J. biol. Chem. 233, 42–8.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, Hokkaido University, Japan

    Sei-Ichi Matsui, Tatsuro Ikeuchi & Samuel H. Hori

Authors
  1. Sei-Ichi Matsui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatsuro Ikeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samuel H. Hori
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsui, SI., Ikeuchi, T. & Hori, S.H. A preliminary note on the hormonal induction of NADP-linked dehydrogenases in phytohaemagglutinin-stimulated human lymphocytes. Histochem J 1, 93–105 (1968). https://doi.org/10.1007/BF01002581

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002581

Keywords

Search

Navigation