Synopsis
The breakdown products resulting from the hydrolysis of chitin by lysozyme stain with Alcian Blue. A method based upon this observation has been developed for the histochemical demonstration of lysozyme activity. The application of this method to the jejunal crypts of several animal species indicates that Paneth cell granules contain lysozyme. The binding of the hydrolysis products with Alcian Blue is so strong that any other alcianophilia (e.g. of acid mucosubstances in goblet cells) can be removed selectively by washing withN-cetylpyridinium chloride and counterstaining with Basic Fuchsin and Nuclear Fast Red.
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Ghoos, Y., Vantrappen, G. The cytochemical localization of lysozyme in Paneth cell granules. Histochem J 3, 175–178 (1971). https://doi.org/10.1007/BF01002560
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DOI: https://doi.org/10.1007/BF01002560