Summary
Using a microdensitometric histochemical assay, Δ5-3β-hydroxysteroid dehydrogenase activity and glucose-6-phosphate dehydrogenase Types I and II hydrogen generation were measured in preovulatory follicles from normal rats, and in follicles from rats given tetrahydrocannabinol for three days prior to sacrifice. Hydroxysteroid dehydrogenase and Type I hydrogen generation are involved in steroidogenesis, whereas Type II hydrogen generation is involved with general cellular metabolism. All ovaries were removed on pro-oestrus, frozen, sectioned and the sections reacted with the appropriate media. Enzyme activity was measured in the theca and in three regions of the membrana granulosa: peripheral antral and corona radiata. Compared to control animals, the hydroxysteroid dehydrogenase activity was significantly reduced in all follicular regions in rats exposed to tetrahydrocannabinol. Type I hydrogen generation was significantly less in the theca and peripheral region of preovulatory follicles from rats given tetrahydrocannabinol, but the same in the antral region and corona radiata. In all follicular regions examined, Type II hydrogen generation was unchanged following tetrahydrocannabinol administration. Thus, only the enzymes specifically associated with follicular steroidogenesis were affected by administration of the drug.
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Adashi, E. Y., Jones, P. B. C. &Hsueh, A. J. W. (1983) Direct antigonadal activity of cannabinoids: suppression of rat granulosa cell functions.Endocr. Metab. 7, E177–85.
Almirez, R. G., Smith, C. G. &Asch, R. H. (1983) The effects of marijuana and Δ9-tetrahydrocannabinol on luteal function in the rhesus monkey.Tert. Steril. 39, 212–7.
Asch, R. H., Smith, C. G., Siler-Khodr, T. M. &Pauerstein, C. J. (1979) Effects of Δ9-tetrahydrocannabinol administration on gonadal steroidogenic activityin vivo.Tert. Steril. 32, 576–82.
Asch, R. H., Smith, C. G., Siler-Khodr, T. M. &Pauerstein, C. J. (1981) Effects of delta-9-tetrahydrocannabinol during the follicular phase of the rhesus monkey (Macaca mulatta).J. Clin. Endocr. Metab. 52, 50–5.
Ayalon, D., Nir, I., Cordova, T., Bauminger, S., Puder, M., Naor, Z., Kashi, R., Zor, U., Harell, A. &Lindner, H. R. (1977) Acute effect of Δ9-tetrahydrocannabinol on the hypothalamo-pituitary-overian axis in the rat.Neuroendocrinology 32, 31–42.
Barraclough, C. A. &Sawyer, C. H. (1955) Inhibition of the release of pituitary ovulatory hormone in the rat by morphine.Endocrinology 57, 329–37.
Burstein, S., Hunter, S. A. &Shoupe, T. S. (1979) Cannabinoid inhibition of rat luteal cell progesterone synthesis.Life Sci. 23, 979–82.
Butcher, R. G. (1972) Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry.Histochemie 32, 171–90.
Chayen, J., Bitensky, L. &Butcher, R. (1973)Practical Histochemistry, pp. 68–75. New York: John Wiley.
Chayen, J., Daly, J. R., Loveridge, N. &Bitensky, L. (1976) The cytochemical bioassay of hormones.Recent Prog. Horm. Res. 32, 33–79.
Daane, T. &Parlow, A. P. (1971) Periovulatory patterns of rat serum follicle stimulating hormone and luteinizing hormone during the normal estrous cycle: effects of pentobarbital.Endocrinology 88, 653–63.
Everett, J. W. &Sawyer, C. H. (1950) A 24-hour periodicity in the “LH-release apparatus” of female rats disclosed by barbiturate sedation.Endocrinology 47, 198–218.
Fortune, J. E. &Armstrong, D. T. (1978) Hormonal control of 17β-estradiol biosynthesis in proestrous rat follicle: estradiol production by isolated theca versus granulosa.Endocrinology 102, 227–35.
Freudenthal, R. I., Martin, J. &Wall, M. E. (1972) Distribution of Δ9-tetrahydrocannabinol in the mouse.Br. J. Pharmac. 44, 244–9.
Husain, S. &Lame, M. W. (1981) Inhibitory effects of Δ9-tetrahydrocannabinol on glycolytic substrates in the rat testis.Pharmacology 23, 102–12.
Jakubovic, A. &McGeer, P. L. (1977) Biochemical changes in rat testicular cellsin vitro product by cannabinoids and alcohol: metabolism and incorporation of labeled glucose, amino acids and nucleic acid precursors.Toxicol. appl. Pharmac. 41, 473–86.
Jakubovic, A., McGeer, E. G. &McGeer, P. L. (1979) Biochemical alterations induced by cannabinoids in the leydig cells of the rat testisin vitro: effects of testosterone and protein synthesis. InMarihuana: Biological Effects (edited byNahas, G. G. andPaton, W. D. M.), pp. 251–64. New York: Pergamon Press.
Moon, Y. S., Duleba, A. J. &Jakubovic, A. (1982) Effect of cannabinoids on progesterone production by ovarian granulosa cells of pig and rat.Life Sci. 31, 315–18.
Nir, I., Ayalon, D., Tsafriri, A., Cordova, T. &Lindner, H. R. (1973) Suppression of the cyclic surge of luteinizing hormone secretion and of ovulation in the rat by Δ9-tetrahydrocannabinol.Nature, Lond. 243, 470–1.
Packman, P. M. &Rothchild, J. A. (1976) Morphine inhibition of ovulation: reversal by naloxone.Endocrinology 99, 7–10.
Purohit, V., Singh, H. H. &Ahluwalia, B. S. (1979) Evidence that the effects of methadone and marihuana on male reproductive organs are mediated at different sites in rats.Biol. Reprod. 20, 1039–44.
Reich, R., Laufer, N., Lewysohn, O., Cordova, T., Ayalon, D. &Tsafriri, A. (1982)In vitro effects of cannabinoids on follicular function in the rat.Biol. Reprod. 27, 223–31.
Samules, L. T., Helmreich, M. L., Lasater, M. B. &Reich, H. (1951) Δ5-3β-hydroxysteroids to α, β-unsaturated ketones.Science 113, 490–1.
Warner, W., Harris, L. S. &Carchman, R. A. (1977) Inhibition of corticosteroidogenesis by delta-9-tetrahydrocannabinol.Endocrinology 101, 1815–20.
Zoller, L. C. &Weisz, I. (1978) Identification of cytochrome P-450, and its distribution in the membrana granulosa of the preovulatory follicle, using quantitative cytochemistry.Endocrinology 103, 310–13.
Zoller, L. C. &Weisz, J. (1979) A quantitative cytochemical study of glucose-6-phosphate dehydrogenase and Δ5-3β-hydroxysteroid dehydrogenase activity in the membrana granulosa of the ovulatory type of follicle of the rat.Histochemistry 62, 125–35.
Zoller, L. C. &Enelow, R. (1983) A quantitative histochemical study of lactate dehydrogenase and succinate dehydrogenase activities in the membrana granulosa of the ovulatory follicle of the rat.Histochem. J. 15, 1055–64.
Zoller, I. C. (1984) A comparison of rat and hamster preovulatory follicles: An examination of differences in morphology and enzyme activity using qualitative and quantitative analyses.Anat. Rec. 210, 279–91.
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Zoller, L.C. Effects of tetrahydrocannabinol on rat preovulatory follicles: a quantitative cytochemical analysis. Histochem J 17, 1347–1358 (1985). https://doi.org/10.1007/BF01002531
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DOI: https://doi.org/10.1007/BF01002531