Abstract
Changes in axonally transported phospholipids of regenerating goldfish optic nerve were studied by intraocular injection of [2-3H]glycerol 9 days and 16 days after nerve crush at 30°C. The four major glycerophospholipids all showed substantial increases in transported radioactivity above non-regenerating controls at both time points, these being maximal (15- to 35-fold) in the optic nerve-tract at 9 days and about half as great at 16 days. In the contralateral optic tectum transported label increased 6- to 13-fold at 9 days and 10- to 25-fold at 16 days in the various glycerophospholipids. While all glycerophospholipids showed absolute increases in both tissues, PS and PI increased relatively more, especially in the tectum. The regeneration-associated increases in transported label of all glycerophospholipids were larger than those previously demonstrated for gangliosides and glycoproteins in the same system.
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Sbaschnig-Agler, M., Ledeen, R.W., Alpert, R.M. et al. Changes in axonal transport of phospholipids in the regenerating goldfish optic system. Neurochem Res 10, 1499–1509 (1985). https://doi.org/10.1007/BF00988862
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DOI: https://doi.org/10.1007/BF00988862