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Age-related changes of glucose metabolism in rat cerebral cortex with reference to glucose-derived amino acids

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Abstract

The parietal cortical slices obtained from 8 week-old (young) and 78 week-old (middle-aged) male Wistar rats were incubated withd-[U-14C]glucose in oxygensaturated Gey's balanced salt solution. Subsequently, the radioactivities of liberated CO2 and glucose-derived amino acids (alanine, aspartate, GABA, glutamate and glutamine) obtained from the slices were measured. In the middle-aged rats as compared to the young rats, the amount of radioactivity of CO2 (P<0.01) and glutamate (P<0.05) showed a significant raduction with glutamine unchanged, while that of alanine (P<0.01), aspartate (P<0.05) and GABA (P<0.05) increased significantly. The results indicate that with advancing age the overall glucose oxidation in the cerebral cortex declines but the metabolic pathway to form amino acids is not uniformly suppressed. Therefore, the above characteristic glucose metabolism could be related to the development of heterogeneous enzyme activities associated with aging in the brain.

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References

  1. Fonnum, F., Lund-Karlsen, R., Maithe-Sorenssen, D., Skrede, K. K., andWalass, I. 1979. Localization of neurotransmitter, particularly glutamate in hypocampus, septum nucleus accumbens and superior colliculus. Prog. Brain Res. 51:167–192.

    PubMed  Google Scholar 

  2. Johnson, J. L. 1978. The excitant amino acids, glutamic and aspartic acids as transmitter candidates in the vertebrate central nervous system. Prog. Neurobiol. 10:155–202.

    Article  PubMed  Google Scholar 

  3. Obata, K., Ito, M., Ochi, R., andSato, M. 1967. Pharmacological properties of the postsynaptic inhibition by Purkinje cell axons and the action of γ-amino butyric acid on Deiters neurons. Exp. Brain Res. 4:43–57.

    Article  PubMed  Google Scholar 

  4. Aprison, M. H., Davidoff, R. H., andWerman, R. 1970. Pages 381–397in A. Lajtha (ed.), Handbook of neurochemistry, Vol. III. Plenum Press, New York.

    Google Scholar 

  5. Dastur, D. K., Lane, M. H., Hansen, D. B., Kety, S. S., Butler, R. N., Perlin, S., andSokoloff, L. 1963. Effects of aging on cerebral circulation and metabolism in man. In Human aging. A biological and behavioral study, edited byJ. E. Birren, R. N. Butler, S. W. Greenhouse, L. Sokoloff andM. R. Yarrow, pages 59–78. US Government Printing Office, Washington, D.C.

    Google Scholar 

  6. Patel, M. S. 1977. Age-dependent changes in oxidative metabolism in rat brain. J. Geront. 32:643–646.

    PubMed  Google Scholar 

  7. Daniel, P. M., Love, E. R., andPratt, O. E. 1978. The effect of age upon the influx of glucose into the brain. J. Physiol. (Lond.) 274:141–148.

    Google Scholar 

  8. Parmacek, M. S., Fox, J. H., Harrison, W. H., Garron, D. C., andSwenie, D. 1979. Effect of aging on brain respiration and carbohydrate metabolism of CBF1, mice. Gerontology 25:185–191.

    PubMed  Google Scholar 

  9. Tyce, G. M., andWong, K.-L. 1980. Conversion of glucose to neurotransmitter amino acids in the brains of young and aging rats. Exp. Geront. 15:527–532.

    Article  Google Scholar 

  10. De Koning-Verest, I. F. 1980. Glutamate metabolism in aging rat brain. Mech. Aging Dev. 13:83–92.

    Article  PubMed  Google Scholar 

  11. Gibson, G. E., Peterson, C., andSansone, J. 1981. Neurotransmitter and carbohydrate metabolism during aging and mild hypoxia Neurobiology of Aging 2:165–172.

    Article  PubMed  Google Scholar 

  12. Kikuchi, S. 1981. Studies on amino acid metabolism of cerebral cortex in experimental uremia with special reference to glutamate metabolism. Sapporo Med. J. 50:127–138.

    Google Scholar 

  13. Smith, C. B., Goochee, C., Rapoport, S. I., andSokoloff, L. 1980. Effects of aging on local rate of cerebral glucose utilization in the rat. Brain 103:351–365.

    PubMed  Google Scholar 

  14. Victoria, J., Andes, A., Satrustegui, J., andMachado, A. 1981. Age-related quantitative changes in enzyme activities of rat brain. Neurochem. Res. 6:127–136.

    Article  PubMed  Google Scholar 

  15. Oeriu, S., Cotescu, G., Teodorescu, O., andSojmu, I. 1962. Carboxylase and transaminase activities and transacetylation capacity in the process of aeging. Studii Cere. Biochem. 5:337–340.

    Google Scholar 

  16. Desmukh, D. R., andPatel, M. S. 1980. Age-dependent changes in glutamate oxidation by nonsynaptic and synaptic mitochondria from rat brain. Mech. Aging Dev. 13:75–81.

    Article  PubMed  Google Scholar 

  17. Benzi, G., Arrigoni, E. Dagani, F., Mazatico, F., Cuti, D., Plogatti, M., andVilla, R. F. 1980. Aging and enzymes, Pages 1–13,in G. Barbagallo-sangiorgi, andA. N. Exton-Smith, (eds.), Plenum, New York.

    Google Scholar 

  18. Matsumoto, H., Kikuchi, S., andIto M. 1982. Age-related changes in the glutamate metabolism of cerebral cortical slices from rats. Neurochem. Res. 7:678–685.

    Article  Google Scholar 

  19. Kanungo, M. S. andKaur, G. 1969. Regulatory changes in enzymes as a function of age of the rat. Proceedings of the 8th International Congress of Gerontology. Vol. I. Pages 622–628.

    Google Scholar 

  20. Epstein, M. H., andBarrow, C. H. JR. 1969. The effects of age on the activity of glutamic acid decarboxylase in various regions of the brain of rats. J. Geront. 24:136–139.

    PubMed  Google Scholar 

  21. Fonda, M. L., Acree, D. W., andAuerbach, S. B. 1973. The relationship of γ-aminobutyrate levels and its metabolism to age in brains of mice. Arch. Biochem. Biophys. 159:622–628.

    Article  Google Scholar 

  22. Mrsulja, B. B., Mršulja, B. J., Cvejić, V., Djuričić, B. M. andRogač, L. J. 1978. Alterations of putative neurotransmitters and enzymes during ischemia in gerbil cerebral cortex. J. Neurol. Transmission, Suppl. 14:23–30.

    Google Scholar 

  23. Gordienko, E. A., Etremova, G. G., andSidorova, N. E. 1975. Effects of age on the brain and livér glutamic synthetase at different media conditions. Pages 244–230,in Molekuljarnye i fiziologicheskie mekhanizmy vozrastnogo razvitija. Naukova Dumka, Kiev.

    Google Scholar 

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Authors and Affiliations

  1. The First Department of Internal Medicine, Sapporo Medical College, South 1 West 16, 060, Sapporo, Hakkaido, Japan

    Hiroyuki Matsumoto, Masaharu Ito, Susumu Kikuchi & Masato Edamura

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  1. Hiroyuki Matsumoto
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  2. Masaharu Ito
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  3. Susumu Kikuchi
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  4. Masato Edamura
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Matsumoto, H., Ito, M., Kikuchi, S. et al. Age-related changes of glucose metabolism in rat cerebral cortex with reference to glucose-derived amino acids. Neurochem Res 10, 1615–1622 (1985). https://doi.org/10.1007/BF00988603

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  • DOI: https://doi.org/10.1007/BF00988603

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