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Chromosome doubling inTurnera ulmifolia (Turneraceae) induced by regeneration of plants from in vitro cultured leaf explants

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Abstract

Plants were regenerated from cultured excised leaf segments ofTurnera ulmifolia (2n = 6x = 30). Cytological studies have demonstrated that chromosome doubling occurred in 100% of the regenerated plants. Probably it was produced by endomitosis, induced by excess of auxins in relation to cytokinins. High bivalent and low quadrivalent frequency, and univalents to octovalents were observed in metaphase I; lagging chromosomes were also found. Probably, the presence of multivalents may be due to the pairing among homoeologous chromosomes because the mother plant is a segmental allohexaploid. The high bivalent frequency may have been caused by preferential pairing of identical chromosomes against the homologous.

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Lavia, G., Fernandez, A. & Marquez, G. Chromosome doubling inTurnera ulmifolia (Turneraceae) induced by regeneration of plants from in vitro cultured leaf explants. Pl Syst Evol 192, 41–48 (1994). https://doi.org/10.1007/BF00985906

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  • DOI: https://doi.org/10.1007/BF00985906

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