Abstract
The characteristics of dopamine uptake after acute and subacute cocaine administration were determined in striata from WKY and SHR. In acutely-treated (40 mg/kg, s.c.) rats, significant increases in the Vmax of dopamine uptake were observed 30 min after the cocaine injection in both strains, without changes in Km values. The in vitro IC50 for cocaine was significantly decreased at 30 min in WKY and at 2 h in SHR. However, the in vitro IC50 for GBR-12909 was significantly increased at 30 min and at 2 h in both strains following cocaine administration. In both strains, the density (Bmax) of the [3H]GBR-12935 binding site was significantly increased at 30 min and at 2 h with no charges in Kd. In subacutely-treated (20 mg/kg, twice daily for 3 or 7 days) rats, a significant increase in the Km for dopamine uptake was observed in 7 day treated SHR. The in vitro IC50 for GBR-12909 was significantly increased in 3 day treated WKY. The results suggest that cocaine administration alters dopamine uptake and characteristics of dopamine uptake sites in the rat brain.
Similar content being viewed by others
References
Dackis, C. A., and M. S. Gold 1985. New concepts in cocaine addiction: the dopamine depletion hypothesis. Neurosci. Biochem. Rev. 9:469–477.
Bhargava, H. N. 1984. Effect of cyclo (leucyl-glycine) on [3H]spiroperidol binding in the corpus striatum and hypothalamus of spontaneously hypertensive rats. Eur. J. Pharmacol. 100:109–112.
Chiu, P., Rajakumar, G., Chiu, S., Kwan, C-Y., and R. K. Mishra. 1982. Enhanced [3H]spiroperidol binding in striatum of spontaneously hypertensive rat (SHR). Eur. J. Pharmacol. 82:243–244.
Le Fur, G., Guilloux, F., Kabouche, M., Mitrani, N., Ferris, O., and A. Uzan. 1981. Central dopaminergic neurons during development of genetic and DOCA-salt hypertension in the rat. Dev. Brain Res. 1:153–163.
Le Fur, G., Guilloux, F., and A. Uzan. 1983. Evidence for an increase in [3H]spiperone binding in hypothalamic nuclei during the development of spontaneous hypertension in the rat. Clin. Exp. Hyperten. A5:1537–1542.
Lim, D. K., Ito, Y., Hoskins, B., Rockhold, R. W., and I. K. Ho. 1989. Comparative studies of muscarinic and dopamine receptors in three strains of rat. Eur. J. Pharmacol. 165:279–287.
Myers, M. M., Whittemore, S. R., and E. D. Hendley. 1981. Changes in catecholamine neuronal uptake and receptor binding in the brains of spontaneously hypertensive rats. Brain Res. 220:325–338.
Van Den Buuse, M., and W. De Jong. 1989. Differential effects of dopaminergic drugs on open-field behavior of spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. J. Pharmacol. Exp. Ther. 248:1189–1196.
Bonnett, J. J., and J. Costentin. 1986. GBR 12783, a potent and selective inhibitor of dopamine uptake: biochemical studies in vivo and ex vivo. Eur. J. Pharmacol. 12:199–209.
Heikkila, R. E., and L. Manzino. 1984. Behavioral properties of GBR 12909, GBR 13069 and GBR 13098: Specific inhibitors of dopamine uptake. Eur. J. Pharmacol. 103:241–248.
Andersen, P. H. 1987. Biochemical and pharmacological characterization of GBR 12935 binding in vitro to rat striatal membranes: labelling of the dopamine uptake complex. J. Neurochem. 48:1887–1896.
Glowinski, J., and L. L. Iversen. 1966. Regional studies of catecholamines in the rat brain-I. The disposition of3H-norepinephrine,3H-dopamine and3H-DOPA in various regions of the rat. J. Neurochem. 13:655–669.
Haberland, N., and L. Hetey. 1987. Studies in postmortem dopamine uptake 1. Kinetic characterization of the synaptosomal dopamine uptake in rat and human brain after postmortem storage and cryopreservation. Comparison with noradrenaline and serotonin uptake. J. Neural Trans. 68:289–301.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and R. J. Randall. 1951. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:265–275.
Munson, P. J., and D. Rodbard. 1980. A versatile computerized approach for the characterization of ligand binding systems. Anal. Biochem. 107:220–239.
Iversen, L. L. 1974. Uptake mechanisms of neurotransmitter amines. Biochem. Pharmacol. 23:1927–1935.
Parenti, M., Flauto, C., Poarati, E. Vescovi, A., and Groppetti, A. 1986. Differential effect of repeated treatment with L-DOPA on dopamine D-1 or D-2 receptors. Neuropharmacol. 25:331–334.
Missale, C., Castelletti, L., Govoni, S., Spano, P. F., Trabucchi, M., and I. Hanbauer. 1985. Dopamine uptake is differentially regulated in rat striatum and nucleus accumbens. J. Neurochem. 45:51–56.
Near, J. A., Bigelow, J. C., and R. M. Wightman. 1988. Comparison of uptake of dopamine in rat striatal chopped tissue and synaptosomes. J. Pharmacol. Exp. Ther. 245:921–927.
Hadfield, M. G., and E. A. Nugent. 1983. Cocaine: comparative effect on dopamine uptake in extrapyramidal and limbic systems. Biochem. Pharmacol. 32:744–746.
Nicolaysen, L. C., Pan, H-T., and J. B. Justice Jr. 1988. Extracellular cocaine and dopamine concentrations are linearly related in rat striatum. Brain Res. 456:317–323.
Tuomisto, J., and L. O. Tuomisto. 1987. Comformationally rigid dopamine analogues as inhibitors of dopamine and 5-hydroxytryptamine uptake by synaptosomes. J. Neural Trans. 68:191–196.
Altar, C. A., and J. F. Marshall. 1988. Neostriatal dopamine uptake and reversal of age-related movement disorders with dopamine uptake inhibitors. Ann. NY Acad. Sci. 515:343–354.
Change, G. D., and V. D. Ramirez. 1989. Studies of the in vitro catabolism of exogenous dopamine as infused through a push-pull cannula implanted in the rat caudate nucleus. Brain Res. 481:265–273.
Sershen, H., Reith, M. E. A., Hashim, A., and A. Lajtha. 1984. Reduction of dopamine uptake and cocaine binding in mouse striatum by N-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Eur. J. Pharmacol. 102:175–178.
Calligaro, D. O., and M. E. Eldefrawi. 1988. High-affinity stereospecific binding of [3H]cocaine in striatum and its relationship to the dopamine transporter. Mem. Biochem. 7:87–106.
Galloway, M. P. 1988. Neurochemical interactions of cocaine with dopaminergic systems. Trend. Pharmacol. Sci. 9:451–454.
Kennedy, L. T., and I. Hanbauer. 1983. Sodium-sensitive cocaine binding to rat striatal membranes: possible relationship to dopamine uptake sites. J. Neurochem. 41:172–178.
Tsuda, K., Tsuda, S., and Y. Masuyama. 1988. The role of dopamine in the regulation of neurotransmitter release in spontaneously hypertensive rats. Jpn. Heart. J. 29:215–222.
Van Den Buuse, M., Versteeg, D. H. G., and W. De Jong. 1986. Brain dopamine depletion by lesions in the substantia nigra attenuates the development of hypertension in the spontaneously hypertensive rat. Brain Res. 365:69–78.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lim, D.K., Yu, Z.J., Hoskins, B. et al. Effects of acute and subacute cocaine administration on the CNS dopaminergic system in Wistar-Kyoto and spontaneously hypertensive rats: III. Dopamine uptake. Neurochem Res 15, 629–634 (1990). https://doi.org/10.1007/BF00973754
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00973754