Abstract
The permeability of Cl− ions through single microdissected plasma membranes from Deiters' neurons was studied by a microtechnique. In particular, the time course of the passage of36Cl− ions from a microchamber, M1, to another one, M2, across the membrane was followed. This study was performed with or without γ-amino-butyric acid (GABA) in the two microchambers. The results suggest that in basal conditions the high intracellular concentration normally present in these neurons, 3.3 mM (1), causes a higher permeability of Cl− in the direction inside → outside in the respect of the plasma membrane. “Extracellular” GABA, 0.1 mM, is able to abolish this imbalance in Cl− permeability in the two opposite directions. This event appears to be the basis for GABA induced hyperpolarization of these neurons.
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Hydén, H., Cupello, A. & Palm, A. “Intracellular” GABA affects the equilibrium distribution of Cl− across the plasma membrane of a GABA acceptive neuron. Neurochem Res 13, 153–157 (1988). https://doi.org/10.1007/BF00973327
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DOI: https://doi.org/10.1007/BF00973327