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Developmental changes in the synthesis of nonhistone nuclear proteins relative to the appearance of a short nucleosomal DNA repeat length in cerebral hemisphere neurons

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Abstract

Fluctuations in the pattern of synthesis of nonhistone nuclear proteins were noted in cerebral hemisphere neurons during early postnatal development of the rat. Noteworthy changes included the synthesis of an acidic nuclear protein of relative molecular weight 41,000 (41 K), two chromatin-associated basic proteins (37 K and 38 K) and several high molecular weight chromatin acidic proteins. These changes in the synthesis of nonhistone nuclear proteins occur at a developmental stage when a short nucleosomal DNA repeat length has appeared in cerebral hemisphere neurons.

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Abbreviations

bp:

base pairs

DTT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

IF:

isoelectric focusing

PMSF:

Phenylmethylsulfonylfluoride

SDS:

sodium dodecyl sulphate

References

  1. Elgin, S. C. R., andWeintraub, H. 1975. Chromosomal proteins and chromatin structures. Ann. Rev. Biochem., 44:726–744.

    Google Scholar 

  2. Kornberg, R. D. 1977. Structure of chromatin. Ann. Rev. Biochem. 46:931–954.

    Google Scholar 

  3. Felsenfeld, C. 1978. chromatin. Nature 271:115–121.

    Google Scholar 

  4. Horgen, P. A., andSilver, J. C. 1978. Chromatin in eukaryotic microbes. Ann. Rev. Microbiol. 32:249–284.

    Google Scholar 

  5. Kornberg, R. D. andKlug, A. 1981. The nucleosome. Sci. Amer. 244:52–64.

    Google Scholar 

  6. Igo-Kemenes, T., Horz, W., andZachau, H. G. 1982. Chromatin. Ann. Rev. Biochem. 51:89–121.

    Google Scholar 

  7. Thomas, J. O., andThompson, R. J. 1977. Variation in chromatin structure in two cell types from the same tissue: A short DNA repeat length in cerebral cortex neurons. Cell 10:633–640.

    Google Scholar 

  8. Ermini, M., andKuenzle, C. C. 1978. The chromatin repeat length of cortical neurons shortens during early postnatal development. FEBS Lett. 90:167–172.

    Google Scholar 

  9. Brown, I. R. 1978. Postnatal appearance of a short DNA repeat length in neurons of the cerebral cortex. Biochem. Biophys. Res. Commun. 84:285–292.

    Google Scholar 

  10. Brown, I. R. 1982. Prenatal appearance of a short nucleosomal DNA repeat length in neurons of the guinea pig cerebral cortex. Biochem. Biophys. Acta 698:307–309.

    Google Scholar 

  11. Whatley, S. A., Hall, C., andLim, L. 1981. Chromatin organization in the rat hypothalamus during early development. Biochem. J. 196:115–119.

    Google Scholar 

  12. Brown, I. R., andGreenwood, P. D. 1982. Chromosomal components in brain cells. Pages 41–69,in Brown, I. R., (ed.), Molecular approaches to neurobiology, Academic Press Inc., New York.

    Google Scholar 

  13. Brown, I. R. 1983. The organization of DNA in brain cells. Pages 217–226,in Lajtha, A. (ed.), Handbook of Neurochemistry Vol. 5, Plenum Publishing Corp., New York.

    Google Scholar 

  14. Greenwood, P. D., Silver, J. C., andBrown, I. R. 1981. Analysis of histones associated with neuronal and glial nuclei exhibiting divergent DNA repeat lengths. J. Neurochem. 37:498–505.

    Google Scholar 

  15. Greenwood, P. D., andBrown, I. R. 1982. Developmental changes in DNase I digestability and RNA template activity of neuronal nuclei relative to the postnatal appearance of a short DNA repeat length. Neurochem. Res. 7:965–976.

    Google Scholar 

  16. Greenwood, P. D., Heikkila, J. J., andBrown, I. R. 1982. Developmental changes in chromatin organization in rat cerebral hemisphere neurons and analysis of DNA reassociation kinetics. Neurochem. Res. 7:525–539.

    Google Scholar 

  17. Gozes, I., Walker, M. D., Kaye, A. M., andLittauer, U. Z. 1977. Synthesis of tubulin and actin by neuronal and glial nuclear preparations from developing rat brain. J. Biol. Chem. 252:1819–1825.

    Google Scholar 

  18. Tsitilou, S. G., Cox, D., Mathias, A. P., andRidge, D. 1979. The characterization of the non-histone chromosomal proteins of the main classes of nuclei from rat brain fractionated by zonal centrifugation. Biochem. J. 177:331–346.

    Google Scholar 

  19. Peters, K. E., andComings, D. E. 1980. Two-dimensional gel electrophoresis of rat liver nuclear washes, nuclear washes and hnRNA proteins. J. Cell Biol. 86:135–155.

    Google Scholar 

  20. Silver, J. C. 1979. Basic nuclear proteins in the aquatic fungusAchyla ambisexualis. Biochim. Biophys. Acta 565:507–516.

    Google Scholar 

  21. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 250:4007–4021.

    Google Scholar 

  22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.

    Google Scholar 

  23. Bonner, W. M., andLaskey, R. A. 1974. A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46:83–88.

    Google Scholar 

  24. Oakley, B. R., Kirsch, D. R., andMorris, N. R. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analyt. Biochem. 105:361–363.

    Google Scholar 

  25. Heizmann, C. W., Arnold, E. M., andKuenzle, C. C. 1980 Fluctuations of non-histone chromosomal proteins in differentiating brain cortex and cerebellar neurons. J. Biol. Chem. 255:11504–11511.

    Google Scholar 

  26. Heizmann, C. W., Arnold, E. M., andKuenzle, C. C. 1982. Changing patterns of single-stranded-DNA-binding proteins in differentiating brain cortex and cerebellar neurons. Eur. J. Biochem. 127:57–61.

    Google Scholar 

  27. Tashiro, T., Mizobe, F., andKurokowa, M. 1974. Characteristics of cerebral non-histone chromatin proteins as revealed by polyacrylamide gel electrophoresis. FEBS Lett. 38:121–124.

    Google Scholar 

  28. Albrecht, J., andHemminki, K., 1976. Chromatin proteins of large brain and small brain nuclei. J. Neurochem. 26:1297–1299.

    Google Scholar 

  29. Burdman, J. A. 1972. The relationship between DNA synthesis and the synthesis of nuclear proteins in rat brain during development. J. Neurochem. 19:1459–1469.

    Google Scholar 

  30. Szijan, I., andBurdman, J. A. 1973. Nuclear proteins in brain of 7-day-old and adult rats. J. Neurochem. 21:1093–1097.

    Google Scholar 

  31. Biesmann, H., andRajewsky, M. F. 1975. Nuclear protein patterns in developing and adult brain and in ethylnitrosourea-induced neuroectodermal tumour of the rat. J. Neurochem. 24:387–393.

    Google Scholar 

  32. Kelly, P. T., andLuttges, M. W. 1976. Mouse brain protein composition during postnatal development: an electrophoretic analysis. J. Neurochem. 27:1163–1172.

    Google Scholar 

  33. Choie, D. D., Friedberg, E. C., Vanden Berg, S. R., andHerman, M. M. 1977. Non-histone chromosomal proteins in mouse brain at different stages of development and in a transplantable mouse teratoma. J. Neurochem. 29:811–817.

    Google Scholar 

  34. Chaudhari, N., andHahn, W. E. 1983. Genetic expression in the developing brain. Science 220:924–928.

    Google Scholar 

  35. Teng, C. S., Teng, C. T., andAllfrey, V. G. 1971. Studies of nuclear acidic proteins. J. Biol. Chem. 246:3597–3609.

    Google Scholar 

  36. Shea, M., andKleinsmith, L. J. 1973. Template-specific stimulation of RNA synthesis by phosphorylated non-histone chromosomal proteins. Biochem. Biophys. Res. Commun. 50:473–477.

    Google Scholar 

  37. Kamiyama, M., Dastuque, B., Defer, N., andKruh, J. 1972. Liver chromatin non-histone proteins. Partial fractionation and mechanism of action on RNA synthesis. Biochim. Biophys. Acta 277:576–583.

    Google Scholar 

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Authors and Affiliations

  1. Department of Zoology, University of Toronto, Scarborough Campus, M1C 1A4, West Hill, Ontario, Canada

    Tina R. Ivanov & Ian R. Brown

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  1. Tina R. Ivanov
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  2. Ian R. Brown
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Ivanov, T.R., Brown, I.R. Developmental changes in the synthesis of nonhistone nuclear proteins relative to the appearance of a short nucleosomal DNA repeat length in cerebral hemisphere neurons. Neurochem Res 9, 1323–1337 (1984). https://doi.org/10.1007/BF00973043

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