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Correlation between active rosette formation and delayed cutaneous hypersensitivity in experimental allergic encephalomyelitis

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Abstract

The effect of encephalitogenic myelin basic protein, BP, on active rosette-forming T cells (ARFC) was compared to that of nonencephalitogenic peptide S42, a synthetic analogue of the tryptophan region of BP. Depression of ARFC by these antigens was reversible within 24 h after a second dose of the antigen into the skin, or after in vitro incubation of lymphocytes with the sensitizing antigen (Ag-ARFC). The ratio of Ag-ARFC to ARFC rose with time following the sensitization but fell shortly before the clinical onset of experimental allergic encephalomyelitis in animals sensitized with BP. In contrast, the Ag-ARFC/ARFC ratios for animals sensitized with peptide S42 reached plateau levels from which they did not drop. The kinetics of the Ag-ARFC/ARFC responses paralleled those for delayed-type skin hypersensitivity (DTH) in the respective animals. The DTH responses rose following sensitization and fell shortly after the appearance of clinical signs of EAE. The results of this study provide in vitro and in vivo evidence for sensitization to myelin basic protein, and focus attention on the ARFC as a measure for an immunologically active cell population which may be quantitated by antigenic stimulation.

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Abbreviations

EAE:

experimental allergic encephalomyelitis

DTH:

delayed-type skin hypersensitivity

ARFC:

active rosette-forming T cells

Ag-ARFC:

antigen-stimulated active rosette-forming T cells

TRFC:

total rosette-forming T cells

References

  1. Hashim, G. A., Lee, D. H., andPierce, J. C. 1977. Antigen stimulated rosette formation by T Lymphocytes in experimental allergic encephalomyelitis.Neurochem. Res. 2:99.

    Google Scholar 

  2. Hashim, G. A. 1975. Non-encephalitogenic antigen-induced suppression and reversal of allergic encephalomyelitis.Nature 256:593.

    Google Scholar 

  3. Hasmin, G. A., Sharpe, R. D., Carvalho, E. F., andStevens, L. E. 1976. Suppression and reversal of allergic encephalomyelitis in guinea pigs with a nonencephalitogenic analogue of the tryptophan region of the myelin basic protein.J. Immunol. 116:126.

    Google Scholar 

  4. Shaw, C. M., Alvord, E. C., Kaku, J., andKies, M. W. 1965. Correlation of experimental allergic encephalomyelitis with delayed type skin sensitivity to specific homologous encephalitogen.Ann. N.Y. Acad. Sci. 122:318.

    Google Scholar 

  5. Hashim, G. A., Hwang, F., andSchilling, F. J. 1973. Experimental allergic encephalomyelitis: Basic protein regions responsible for delayed hypersensitivity.Arch. Biochem. Biophys. 156:298.

    Google Scholar 

  6. Eylar, E. H., Brostoff, S., Hashim, G. A., Caccam, J., andBurnett, P. 1971. Basic A1 protein of the myelin membrane.J. Biol. Chem. 246:5770.

    Google Scholar 

  7. Felsburg, P. J., Edelman, R., andGilman, R. H. 1976. Active E rosette test: Correlation with delayed cutaneous hypersensitivity.J. Immunol. 116:1110.

    Google Scholar 

  8. Wybran, J., andFudenberg, H. H. 1971. Rosette formation, a test for cellular immunity.Trans. Assoc. Am. Physicians 84:239.

    Google Scholar 

  9. Wybran, J., Carr, M. C., andFudenberg, H. H. 1972. The human rosette-forming cell as a marker of a population of thymus-derived cells.J. Clin. Invest. 51:2537.

    Google Scholar 

  10. Wybran, J., Levin, A. S., Spitler, L. E., andFudenberg, H. H. 1973. Rosetteforming cells, immunologic deficiency diseases and transfer factor.New Engl. J. Med. 288:710.

    Google Scholar 

  11. Wybran, J., andFudenberg, H. H. 1973. Thymus-derived rosette-forming cells in various human disease states: Cancer, lymphoma, bacterial and viral infections, and other diseases.J. Clin. Invest. 52:1026.

    Google Scholar 

  12. Fudenberg, H. H., Wybran, J., andRobbins, D. 1975. T-rosette-forming cells, cellular immunity and cancer.New Engl. J. Med. 292:475.

    Google Scholar 

  13. Yu, D. T., Clements, P. L., Peter, J. B., Levy, J., Paulus, H. E., andBarnett, E. V. 1974. Lymphocyte characteristics in rheumatic patients and the effect of azothioprine therapy.Arthritis Rheum. 17:37.

    Google Scholar 

  14. Gupta, S., Grieco, M. H., andSigel, I. 1975. Suppression of T-lymphocyte rosettes by rifampin. Studies in normals and patients with tuberculosis.Ann. Intern. Med. 82:484.

    Google Scholar 

  15. Gupta, S., Grieco, M. H., andCushman, P. 1974. Impairment of rosette-forming T lymphocytes in chronic marijuana smokers.New Engl. J. Med. 291:874.

    Google Scholar 

  16. Cushman, P., Khurana, R., andHashim, G. 1975. Marijuana and rosette formation of T lymphocytes.In Szaraz, S., andBraude, M. (eds.), Pharmacology of Cannabis, Raven Press, New York.

    Google Scholar 

  17. Berenyi, M. R., Straus, B., andAvila, L. 1975. T rosettes in alcoholic cirrhosis of the liver.J. Am. Med. Assoc. 232:44.

    Google Scholar 

  18. Yu, D. T. Y. 1975. Human lymphocyte subpopulation: Early and late rosettes.J. Immunol. 115:91.

    Google Scholar 

  19. Levine, S., Hoenig, E. M., andWenk, E. J. 1967. Altered distribution of lesions after repeated passive transfers of allergic encephalomyelitis.Proc. Soc. Exp. Biol. Med. 126:454.

    Google Scholar 

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Authors and Affiliations

  1. Departments of Surgery and Microbiology, St. Luke's Hospital Center and Columbia University, Amsterdam Avenue at 114th Street, 10025, New York, New York

    Duk H. Lee, George A. Hashim & James C. Pierce

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  1. Duk H. Lee
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  2. George A. Hashim
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  3. James C. Pierce
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Lee, D.H., Hashim, G.A. & Pierce, J.C. Correlation between active rosette formation and delayed cutaneous hypersensitivity in experimental allergic encephalomyelitis. Neurochem Res 2, 293–302 (1977). https://doi.org/10.1007/BF00969359

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