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Inhibition of transporter mediated γ-aminobutyric acid (GABA) release by SKF 89976-A, a GABA uptake inhibitor, studied in a primary neuronal culture from chicken

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Abstract

The effect of SKF 89976-A, a lipophilic non-substrate inhibitor of the γ-aminobutyric acid (GABA) transporter, on the release of radioactive GABA andd-aspartate has been studied. Neuronal cultures from 8 day old chick embryos, grown for six days, served as a model. The cultures were incubated with [3H]d-aspartate and [14C] GABA with the subsequent addition of high or low concentrations of SKF 89976-A. Finally the cultures were exposed to differently composed media for either 30 or 300 seconds. The release was quantified, using liquid scintillation counting. The efflux of [3H]d-aspartate and [14C] GABA was increased by [K+] and time, and a minimum value was obtained at [Ca2+] 1.05 mM. The release of both [3H]d-aspartate and [14C] GABA was inhibited by SKF 89976-A. The obtained results indicate that transporter mediated processes are the major mechanisms of transmitter release in the investigated model.

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Authors and Affiliations

  1. Dept. of Zoophysiology, University of Umeå, S-901 87, Sweden

    Lena Lewin, Mats-Olof Mattsson & Åke Sellström

  2. Swedish Defence Research Establishment, S-901 82, Sweden

    Åke Sellström

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  1. Lena Lewin
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  2. Mats-Olof Mattsson
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  3. Åke Sellström
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Lewin, L., Mattsson, MO. & Sellström, Å. Inhibition of transporter mediated γ-aminobutyric acid (GABA) release by SKF 89976-A, a GABA uptake inhibitor, studied in a primary neuronal culture from chicken. Neurochem Res 17, 577–584 (1992). https://doi.org/10.1007/BF00968786

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