Abstract
The rat retina and the different brain regions contain membranes sites that bindl-lysine in the nanomolar range. These binding sites undergo changes in different experimental conditions, thus: I) intraocular injection of kainic acid induces a reduction of the density ofl-lysine binding sites, II)d,l-α-aminoadipic acid injected into the eye enhances both kinetic parameters (B max andK d) ofl-[3H]lysine binding sites, III) the intraperitoneal injection of iodoacetic acid decreases the sensitivity for its ligand binding sites, and IV) the exposure to darkness of the rats reducesl-[3H]lysine binding in the retina, thalamus, hypothalamus and superior colliculus, but not in the occipital cortex; such a decrease appears to be characterized, at least in the retina, by a lower sensitivity of the binding sites forl-lysine after the exposure to darkness. The results show thatl-lysine binding sites are located on kainic acid-sensitive cells and can be involved in the physiological mechanism of vision.
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Piccoli, F., Guarneri, P., Guarneri, R., andPonte, F. 1986.l-[3H]lysine binding to rat retinal membrane. I. Quantitative determination and characterization of the binding sites. neurochem. Res. 11:
Pasantes-Morales, H., Klethi, J., Ledig, M., andMandel, P. 1972. Free amino acids of chicken and rat retina. Brain Res. 41:494–497.
Ehinger, B. 1972. Cellular location of the uptake of some amino acids into the rabbit retina. Brain Res. 46:297–311.
Coyle, J. T., Biziere, K., andSchwarcz, R. 1978. Neurotoxicity of excitatory amino acids in the neural retina. Pages 177–188, inMcGeer, E. G., Olney, J. W., andMcGeer, P. L. (eds.), Kainic acid as a tool in Neurobiology, Raven Press, New York.
Yazulla, S. andKleinschmidt, J. 1980. The effects of intraocular injection of kainic acid on the synaptic organization of the goldfish retina. Brain Res. 182:287–301.
Pedersen, O., andKarlsen, R. L. 1979. Destruction of Muller cells in the adult rat by intravitreal injection ofd,l-α-aminoadipic acid. An electron microscopic study. Exp. Eye Res. 28:569–575.
Bonaventure, N., Rouseel, G., andWioland, N. 1981. Effects ofd,l-α-aminoadipic acid on Muller cells in frog and chicken retinae in vivo: relation to ERG b wave, ganglion cell discharge and tectal evoked potentials. Neurosci. Lett. 27:81–87.
Orzalesi, N., Calabria, G. A., andGrignolo, A. 1970. Experimental degeneration of the rabbit retina induced by iodoacetic acid. Exp. Eye Res. 9:246–253.
Potts, A. M. 1965. Selective action of chemical agents on individual retinal layers. Pages 155–162,in Graymore, C. N. (ed.), Biochemistry of the retina, Academic Press, London.
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Piccoli, F., Guarneri, P., Guarneri, R. et al. l-[3H]lysine binding to rat retinal membrane: II. effect of kainic acid,d,l-α-aminoadipic acid, iodoacetic acid, and modification by dark-exposure. Neurochem Res 11, 1719–1726 (1986). https://doi.org/10.1007/BF00967750
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DOI: https://doi.org/10.1007/BF00967750