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Hexose transport by brain slices: Further studies on energy dependence

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Abstract

We studied the uptake of [3H]2-deoxyglucose ([3H]2DG) by slices of rat cerebral cortex in vitro as a model of glucose transport by brain. Slices were incubated with [3H]2DG, or withl-[3H]glucose as a marker for diffusion; the difference between [3H]2DG uptake andl-[3H]glucose uptake was defined as net [3H]2DG transport. Net [3H]2DG transport was a function of incubation temperature, with an estimated temperature coefficient of 1.87 from 15°C to 25°C. The net uptake of [3H]2DG was not inhibited by phlorizin or phloretin in concentrations well above the reported Ki of these inhibitors for hexose uptake in other systems. To examine the hypothesis that [3H]2DG transport by brain slices is dependent on mitochondrial energy, we studied net [3H]2DG uptake by slices which had been preincubated in media designed to alter intracellular ATP stores. The transport process was very sensitive to inhibition by DNP, but the correlation between [3H]2DG transport and ATP levels was unclear. In contrast to our published hypothesis that the transport process required mitochondrial energy, these data indicate that dependence on energy is not absolute.

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Authors and Affiliations

  1. Department of Pharmacology, Uniformed Services University, 4301 Jones Bridge Road, 20814, Bethesda, Maryland

    Jayne Kyle-Lillegard & Barry I. Gold

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  1. Jayne Kyle-Lillegard
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  2. Barry I. Gold
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Kyle-Lillegard, J., Gold, B.I. Hexose transport by brain slices: Further studies on energy dependence. Neurochem Res 8, 473–481 (1983). https://doi.org/10.1007/BF00965103

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  • DOI: https://doi.org/10.1007/BF00965103

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