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Development of [3H]muscimol binding to subcellular particles of a culture of mouse brain

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Abstract

“Specific” binding of [3H]muscimol (11.5 nM), related to GABA receptors, occurred in subcellular particles prepared from 4-, 8-, and 12-day cultures of embryonic (14-to 15-day-old) mouse cerebrum, but not to particles prepared from 20-to 45-day cultures. Phase-contrast microscopy revealed that the presence of neuronal elements (neuroblasts, neurites) paralleled the binding of [3H]muscimol. Further evidence is thus provided for using high-affinity [3H]muscimol binding as a “neuronal marker.”

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Author notes

  1. V. Varga

    Present address: 1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary

Authors and Affiliations

  1. Centre de Neurochimie du CNRS, Faculté de Médecine, 67085, Strasbourg Cedex, France

    L. L. Sarliève, F. V. DeFeudis, L. Ossola & V. Varga

  2. Institute de Chimie Biologique, Faculté de Médecine, 67085, Strasbourg Cedex, France

    L. L. Sarliève, F. V. DeFeudis, L. Ossola & V. Varga

Authors
  1. L. L. Sarliève
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  2. F. V. DeFeudis
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  3. L. Ossola
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  4. V. Varga
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  5. P. Mandel
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Supported by a UNESCO/IBRO fellowship

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Sarliève, L.L., DeFeudis, F.V., Ossola, L. et al. Development of [3H]muscimol binding to subcellular particles of a culture of mouse brain. Neurochem Res 5, 1279–1290 (1980). https://doi.org/10.1007/BF00964963

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  • DOI: https://doi.org/10.1007/BF00964963

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