Skip to main content
SpringerLink
Log in

Glycoproteins and glycopeptides in the chromaffin granule membrane

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

The glycoproteins and glycopeptides of adrenal chromaffin granule membranes have been analyzed by gel filtration, electrophoresis, and amino acid analysis. It has been found that almost all of the polypeptides present in the membrane are glycoproteins. Indeed, most of these possess sugar specificities which permit binding to concanavalin A-Sepharose. A new set of low-molecular-weight glycopeptides was found. There is an inverse correlation between carbohydrate content and polypeptide molecular weight.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Winkler, H. 1976. The composition of adrenal chromaffin granules: An assessment of controversial results. Neuroscience 1:65–80.

    Google Scholar 

  2. Hogue-Angeletti, R. A., Roda, L. G., Nolan, J. A., andZaremba, S. 1980.In Schneider, D., andBradshaw, R. A. (eds.) Proteins of the Nervous System, second edition, Raven Press, New York (in press).

    Google Scholar 

  3. Zinder, O., Hoffman, P. G., Bonner, W. M., andPollard, H. B. 1978. Catecholamine storage vesicles. Cell Tissue Res. 188:153–170.

    Google Scholar 

  4. Huber, E., Konig, P., Schuler, G., Aberer, W., Plattner, H., andWinkler, H. 1979. Characterization and topography of the glycoproteins of adrenal chromaffin granules. J. Neurochem. 32:35–47.

    Google Scholar 

  5. Cahill, A. L. andMorris, S. J. 1979. Soluble and membrane lectin-binding glycoproteins of the chromaffin granule. J. Neurochem. 32:855–867.

    Google Scholar 

  6. Bartlett, S. F., andSmith, A. D. 1974. Adrenal chromaffin granules: Isolation and disassembly. Methods Enzymol. 31:379–389.

    Google Scholar 

  7. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., andSmith, F. 1956. Determination of carbohydrate by a phenol-sulfuric acid method. Anal. Chem. 28:350–362.

    Google Scholar 

  8. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–687.

    Google Scholar 

  9. Roda, L. G., andHogue-Angeletti, R. A. 1979. Peptides in the adrenal medulla chromaffin granule. FEBS Lett. 107:393–397.

    Google Scholar 

Download references

Author information

Author notes

  1. L. Giorgio Roda

    Present address: Department of Pharmacology, University of Ancona, Ancona, Italy

Authors and Affiliations

  1. Laboratory of Neuropathology Department of Pathology, University of Pennsylvania School of Medicine, 19104, Philadelphia, Pennsylvania

    L. Giorgio Roda, Emily P. Slater & Ruth A. Hogue-Angeletti (recipient of a Research Career Development Award, NS-00201)

Authors
  1. L. Giorgio Roda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emily P. Slater
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruth A. Hogue-Angeletti
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was supported by grant NS-13201 from the National Institutes of Health.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roda, L.G., Slater, E.P. & Hogue-Angeletti, R.A. Glycoproteins and glycopeptides in the chromaffin granule membrane. Neurochem Res 5, 1243–1250 (1980). https://doi.org/10.1007/BF00964960

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00964960

Keywords

Search

Navigation