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Differential inhibition of brain specific [3H]flunitrazepam binding by several types of dyes

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Abstract

Several dyes, representing different structural classes, inhibit [3H]flunitrazepam binding to brain specific receptors in the rat with 50% inhibition in the 1 to 100 μM range. Crystal Violet and Methyl Violet 2B inhibited more potently in the forebrain than in the cerebellum. Congo Red yielded a Hill number near 2.3, probably reflecting positive cooperativity between interacting binding sites in benzodiazepine receptor complexes. Toluidine Blue 0 was the most potent of the dyes tested (IC50=1 μM in cerebellum) and inhibited more potently in cerebellum than in forebrain.

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Authors and Affiliations

  1. Rockland Research Institute, 10962, Orangeburg, New York

    Thomas M. Smith & Richard F. Squires

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  1. Thomas M. Smith
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  2. Richard F. Squires
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Smith, T.M., Squires, R.F. Differential inhibition of brain specific [3H]flunitrazepam binding by several types of dyes. Neurochem Res 8, 1177–1183 (1983). https://doi.org/10.1007/BF00964931

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