Abstract
The frontal cortex, hippocampus, and cerebellum of the Fischer 344 rat were examined for an age-dependent change in neuronal and non-neuronal binding. Clonazepam and Ro5-6669 displaceable [3H]diazepam binding were used as indicators of [3H]diazepam binding on neuronal and non-neuronal membranes, respectively. In both the frontal cortex and the hippocampus, clonazepam displaceable [3H]diazepam binding in the senescent rat was significantly less than the young and mature rat. In the frontal cortex, Ro5-6669 did not significantly displace [3H]diazepam binding in any age group. The Ro5-6669 displaceable [3H]diazepam binding in the hippocampus was not altered with age. In the cerebellum clonazepam and Ro5-6669 displaceable binding in the old rat was significantly less and more, respectively, compared to the young rat.
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Giles, H. G., MacLeod, S. M., Wright, J. R., andSellers, E. M. 1978. Influence of age and previous use on diazepam dosage required for endoscopy. Can. Med. Assoc. J. 118:513–514.
Reidenberg, M. M., Levy, M., Warner, H., Countinho, C. B., Schwartz, M. A., Yu, G., andCheripko, J. 1978. Relationship between diazepam dose, plasma level, age and central nervous system depression. Clin. Pharmacol. Ther. 23:371–374.
Memo, M., Spano, P. F., andTrabucchi, M. 1981. Brain benzodiazepine receptor changes during ageing. J. Pharm. Pharmacol. 33:64.
Calderini, G., Bonetti, A. C., Aldinio, A., Savoini, G., Diperri, B., Biggio, G., andToffano, G. 1981. Functional interaction between benzodiazepine and GABA recognition sites in aged rats. Neurobiol. Aging 2:309–313.
Pedigo, N. W., Schoemaker, H., Morelli, M., McDougal, J. N., Malick, J. B., Burks, T. F., andYamamura, H. I. 1981. Benzodiazepine receptor binding in young, mature and senescent rat brain and kidney. Neurobiol. Aging 2:83–88.
Heusner, J. E., andBosmann, H. B. 1981. GABA stimulation of [3H]diazepam binding in aged mice. Life Sci. 29:971–974.
DeBlasi, A., Cotecchia, S., andMennini, T. 1982. Selective changes of receptor binding in brain regions of aged rats. Life Sci. 31:335–340.
Gallager, D. W., Mallorga, P., Oertel, W., Henneberry, R., andTallman, J. 1981. [3H]diazepam binding in mammalian central nervous system: A pharmacological characterization. J. Neurosci. 1:218–225.
Marangos, P. J., Patel, J., Boulenger, J. P., andRosenberg, R. C. 1982. Characterization of peripheral-type benzodiazepine binding sites in brain using [3H]Ro5-4864. Mol. Pharmacol. 22:26–32.
Richards, J. G., Mohler, H., andHaefely, W. 1982. Benzodiazepine binding sites: receptors or acceptors? Trends in Pharmacol. Sci. 3:233–235.
Hullihan, J. P., Spector, S., Taniguichi, T., andWang, J. K. 1983. The binding of [3H]diazepam to guinea-pig ileal longitudinal muscle and in vitro inhibition of contraction by benzodiazepines. Br. J. Pharmacol. 78:321–327.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Steel, R., andTorrie, J. 1980. Principles and Procedures of Statistics, pages 187–188, McGraw-Hill Book Co., New York.
Brody, H. 1955. Organization of the cerebral cortex. III. A study of aging in the human cerebral cortex. J. Comp. Neurol. 102:511–556.
Shefer, V. F. 1976. The pathology of the ippocampus as one of the possible factors in the pathogenesis of some dementia of old age. Zh. Nevopatol. Psikhiatr. 76:1032–1036.
Brody, H. 1981. Biological mechanisms in aging. Pages 563–566,in Schimke, R. T. (ed.), NIH Publication No. 81-2194, Bethesda.
Feldman, M. L., andDowd, C. 1975. Loss of dendritic spines in aging cerebral cortex. Anat. Embryol. 148:279–301.
Glick, R., andBondareff, W. 1979. Loss of synapses in the cerebellar cortex of the senescent rat. J. Gerontol. 36:818–822.
Machado-Salas, J. P., andSchiebel, A. B. 1979. Limbic system of the aged mouse. Exp. Neurol. 63:347–355.
Artukhina, N. I. 1968. Electron microscopic study of aged changes of synapses in brain cortex in rats. Tsitologiia 10:1505–1513.
Geinisman, Y. 1979. Loss of axosomatic synapses in the dentate gyrus of aged rats. Brain Res. 168:485–492.
Bolis, C. L. 1982. Neuron-glia interaction and aging. Pages 99–113,in Terry, R. D., Bolis, C. L., andToffano, G. (eds.) Neural Aging and Its Implications in Human Neurological Pathology (Aging Vol. 18) Raven Press, New York.
Vaughen, D. W., andPeters, A. 1974. Neuroglial cells in the cerebral cortex of rats from young adulthood to old age: An electron microscope study. J. Neurocytol. 3:405–429.
Diamond, M. C., Johnson, R. E., andGold, M. W. 1977. Changes in neuron number and size and glia number in the young, adult, and aging rat medial occipital cortex. Behav. Biol. 20:409–418.
Tallman, J. F., Thomas, J. W., andGallager, D. W. 1978. GABA ergic modulation of benzodiazepine binding site sensitivity. Nature 274:383–385.
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Komiskey, H.L., MacFarlan, M.F. Aging: Effect on neuronal and non-neuronal benzodiazepine binding sites. Neurochem Res 8, 1135–1141 (1983). https://doi.org/10.1007/BF00964927
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DOI: https://doi.org/10.1007/BF00964927